We narrowed to 7,460 results for: Lif

1. hARL15_32-197

   Plasmid

   #209799

   Purpose

   Bacterial expression of human ARL15 GTPase domain

   Depositor

   Kalle Gehring

   Article

   Mahbub et al Elife. 2023 Jul 14;12:e86129. doi: 10.75

   Insert

   ARL15 (ARL15 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 32-197 only

   Promoter

   T7

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mAMCase_CatD_6xHis_pcDNA3.1

   Plasmid

   #200229

   Purpose

   Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

   Depositor

   James S Fraser

   Article

   Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

   Insert

   Acidic Mammalian Chitinase (Chia1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S354F

   Promoter

   CMV

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-RO1.7-Hsp90ab1-FLAG

   Plasmid

   #206355

   Purpose

   AAV plasmid expressing FLAG-tagged HSP90AB1 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

   Insert

   Hsp90ab1 (Hsp90ab1 Mouse)
   

   Use

   AAV

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   1.7kb human red opsin

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-mCherry-SPASTIN, L177D

   Plasmid

   #180649

   Purpose

   Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Starts at M87, L177D mutation, has siRNA resistan…

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-THTAP-VPS9D1, residues 1-97

   Plasmid

   #180640

   Purpose

   Bacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   VPS9D1 (C16orf7 Human)
   

   Use

   Tags

   HIS-GST

   Expression

   Bacterial

   Mutation

   Residues 1-97

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Myc-KATNB1

   Plasmid

   #160054

   Purpose

   Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNB1 (KATNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 KATNA1 MIT residues 1-79

   Plasmid

   #180614

   Purpose

   Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-79

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 KATNAL1 MIT residues 1-87

   Plasmid

   #180618

   Purpose

   Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNAL1 (KATNAL1 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-87

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA528 KATNAL2 residues 1-98

   Plasmid

   #180619

   Purpose

   Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNAL2 (KATNAL2 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-98

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCA528 RPS6KC1 MIT, residues 235-316

    Plasmid

    #180622

    Purpose

    Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    RPS6KC1 (RPS6KC1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 235-316

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

    Plasmid

    #197421

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

    Plasmid

    #197422

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

    Plasmid

    #197423

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

    Plasmid

    #197424

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

    Plasmid

    #197425

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-guide-puro mCsnk2b - 1

    Plasmid

    #198505

    Purpose

    lentiviral stable expression of mCsnk2b gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mCsnk2b (Csnk2b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-guide-puro mCsnk2b - 2

    Plasmid

    #198506

    Purpose

    lentiviral stable expression of mCsnk2b gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mCsnk2b (Csnk2b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. siRNA resistant ABHD16A-S176AS306A-mNG

    Plasmid

    #195165

    Purpose

    mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    ABHD16A (ABHD16A Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Wobble mutations for siRNA resistance from L90 to…

    Promoter

    CMV

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. siRNA-resistant ABHD16A-H189AD194A-mNG

    Plasmid

    #195166

    Purpose

    mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    ABHD16A (ABHD16A Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Wobble mutations for siRNA resistance from L90 to…

    Promoter

    CMV

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. siRNA-resistant ABHD16A-S355A-mNG

    Plasmid

    #195167

    Purpose

    mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    ABHD16A (ABHD16A Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Wobble mutations for siRNA resistance from L90 to…

    Promoter

    CMV

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. siRNA-resistant ABHD16A-H189AD194A-Halo

    Plasmid

    #195168

    Purpose

    mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    ABHD16A (ABHD16A Human)
    

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Wobble mutations for siRNA resistance from L90 to…

    Promoter

    CMV

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. siRNA-resistant ABHD16A-S355A-Halo

    Plasmid

    #195169

    Purpose

    mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    ABHD16A (ABHD16A Human)
    

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Wobble mutations for siRNA resistance from L90 to…

    Promoter

    CMV

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL4.15_pT-REX17

    Plasmid

    #195497

    Purpose

    Firefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Promoter region of lncRNA T-REX17
    

    Use

    Tags

    luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. UAS-dsarm(E,K450R)

    Plasmid

    #187881

    Purpose

    Gal4/UAS expression of dSarm(isoE,K450R)

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    dSarm isoform E (Sarm )
    

    Use

    Tags

    Expression

    Mutation

    K450R

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP

    Plasmid

    #190039

    Purpose

    Expression of FIP200 mutant

    Depositor

    James Hurley

    Article

    Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    GST and MBP

    Expression

    Mammalian

    Mutation

    (641-779)deletion & 1363 RDKDLIES 1370 to GSS…

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFUGW-shRIIα

    Plasmid

    #183454

    Purpose

    sgRNA targeting rat PKA-RIIα subunits

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p8322 pDONR ATG-YAP1 S94A-Stop

    Plasmid

    #184526

    Purpose

    Subcloning YAP1 S94A

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 S94A (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    S94A

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p8321 pDONR ATG-YAP1 S127A-Stop

    Plasmid

    #184525

    Purpose

    Subcloning YAP1 S127A

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 S127A (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    S127A

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJET-50-DExogron-mCherry-R11b

    Plasmid

    #179904

    Purpose

    Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJET-23-DExCon-mCherry-R11b

    Plasmid

    #179898

    Purpose

    Donor with homologous arms for Rab11b to knock in DExCon-mCherry module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge