Skip to main content
Addgene
Showing: 2611 - 2640 of 22251 results

  1. iFluoxSnFR

    Plasmid
    #182808
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iFluoxSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182808-plasmid-map-sequence-id-358173

    Enlarge

  3. pSLiP-G1G2

    Plasmid
    #188965
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    188965-plasmid-map-sequence-id-374348

    Enlarge

  7. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

    Enlarge

  10. pcDNA-HA-PfROM4 (G470A/G471N)

    Plasmid
    #154859
    Purpose
    Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfROM4 (PF3D7_0506900 Synthetic)
    Use
    Tags
    triple HA
    Expression
    Mammalian
    Mutation
    Glycine 470 changed to Alanine and Glycine 471 ch…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154859-plasmid-map-sequence-id-310420

    Enlarge

  11. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid
    #154873
    Purpose
    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfROM1 (PF3D7_0506900 Synthetic)
    Use
    Tags
    triple HA
    Expression
    Mammalian
    Mutation
    Alanine 144 changed to Glycine and Asparagine 145…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154873-plasmid-map-sequence-id-308089

    Enlarge

  21. p5E-161K enhancer-NR2F2-MUT

    Plasmid
    #138371
    Purpose
    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated
    Depositor
    Nathan Lawson
    Article
    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC
    Insert
    161k human enhancer with conserved NR2F2 binding sites mutated
    Use
    Gateway compatible
    Tags
    Expression
    Mutation
    NR2F2 sites mutated
    Promoter
    none
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    138371-plasmid-map-sequence-id-272006

    Enlarge

  22. p5E-161K enhancer-ETS-CORE-MUT

    Plasmid
    #138372
    Purpose
    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated
    Depositor
    Nathan Lawson
    Article
    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC
    Insert
    human 161K human enhancer with ETS bind sites mutated
    Use
    Gateway compatible
    Tags
    Expression
    Mutation
    ETS factor binding sites mutated
    Promoter
    NONE
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    138372-plasmid-map-sequence-id-272008

    Enlarge

Showing: 2611 - 2640 of 22251 results