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pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-Puro

Plasmid

#211623

Purpose

Rhno1 S162A

Depositor

Agnel Sfeir

Publication

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S162A

Promoter

CMV

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-Puro

Plasmid

#211625

Purpose

Rhno1 S164A

Depositor

Agnel Sfeir

Publication

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S164A

Promoter

CMV

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-Puro

Plasmid

#211626

Purpose

Rhno1 S178A

Depositor

Agnel Sfeir

Publication

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S178A

Promoter

CMV

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 SR3

Plasmid

#192547

Purpose

pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Publication

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)

Use

Gateway Cloning

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291D

Plasmid

#115193

Purpose

Lentiviral transduction and expression of PDHA2S291D into any mammalian cell

Depositor

Mohan Babu

Publication

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291D

Promoter

EF1alpha

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-ABL1-gRNA1-exon4

Plasmid

#163322

Purpose

eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Publication

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 1_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)-ABL1-gRNA2-exon4

Plasmid

#163323

Purpose

eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Publication

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 2_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pUC19 KGA HA5'-mKO2-3'

Plasmid

#110406

Purpose

Homology donor for glutaminase KGA isoform knock-in, mKO2

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Promoter

none

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGDF11 #2

Plasmid

#83084

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pJF6

Plasmid

#21678

Depositor

James Haber

Publication

Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Mutation

HO digestion site in one of the LacZ genes

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pMSCV-ALDH1A1

Plasmid

#260552

Purpose

Expressing human ALDH1A1 using the pMSCVpuro retroviral vector is a standard, robust method for generating stable overexpressing cell lines

Depositor

Paola Marcato

Publication

Marcato et al Mol Oncol. 2015 Jan;9(1):17-31. doi: 10.

Insert

ALDH1A1 coding sequence (ALDH1A1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Aug. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO H2BmRFP-2A-Plgrkt Δ139-147 v4 trcr filler

Plasmid

#256784

Purpose

lentiviral construct with H2BmRFP-2A-Plgrkt (mouse) Δ139-147 and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.

Depositor

Daniel Schramek

Publication

Al-Zahrani et al Nature. 2026 Jul 8. doi: 10.1038/s41586-

Insert

Plgrkt (Plgrkt Mouse)

Use

Lentiviral

Mutation

delta139-147

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO.1-puro-shCHD1_A

Plasmid

#247237

Purpose

shRNA knockdown of human CHD1

Depositor

Miguel Ramalho-Santos

Publication

Cho et al Oncogene. 2026 Apr;45(13):1198-1210. doi

Insert

shRNA targeting human CHD1 (CHD1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO.1-puro-shCHD1_B

Plasmid

#247238

Purpose

shRNA knockdown of human CHD1

Depositor

Miguel Ramalho-Santos

Publication

Cho et al Oncogene. 2026 Apr;45(13):1198-1210. doi

Insert

shRNA targeting human CHD1 (CHD1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2-mCherry sgmRELA_2

Plasmid

#241453

Purpose

Delete mouse Rela

Depositor

Richard Possemato

Insert

sgmRELA_2 (RELA Synthetic)

Use

CRISPR

Expression

Mammalian

Available since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-Myc-TRIP12-SB-Hygro

Plasmid

#216772

Purpose

Myc-TRIP12-SB; the substrate binding (SB) domain (AA 1-1650) of TRIP12 with an N-terminal Myc-tag & a hygromycin resistance cassette

Depositor

Robert Sobol

Publication

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Myc-TRIP12-SB (TRIP12 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR-Flag-Polb(TM)-C

Plasmid

#216775

Purpose

Flag-Polb(TM), C-terminal domain (AA 91-335); TM = L301R/V303R/V306R

Depositor

Robert Sobol

Publication

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Flag-Polb (POLB Human)

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain (amino acids 91-335); TM = L301…

Available since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 Hygro sgRELB_2

Plasmid

#241448

Purpose

Delete RELB

Depositor

Richard Possemato

Insert

sgRELB_2 (RELB Synthetic)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 Hygro sgRELA_1

Plasmid

#241445

Purpose

Delete RELA

Depositor

Richard Possemato

Insert

sgRELA_1 (RELA Synthetic)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 Hygro sgRELB_1

Plasmid

#241452

Purpose

Delete RELB

Depositor

Richard Possemato

Insert

sgRELB_1 (RELB Synthetic)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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