We narrowed to 4,720 results for: donor
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Plasmid#157784Purposedonor plasmid for C-terminal FLAG tag fusion of human FMR1DepositorAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only
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HES7-NLuc-2A-tdTomato
Plasmid#130932PurposeDonor vector for tagging human HES7 C-terminus with NanoLuc and tdTomatoDepositorAvailable SinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbG-APRT-J-u32uni-PdTKmCh
Plasmid#107279PurposeAPRT c.407T>C (APRT*J) donor vector with unilateral microhomologyDepositorInsertsMutationc.402A>T, c.407T>CAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAT-PB-CG2APtk
Plasmid#86003Purposehomology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassetteDepositorInserteGFP_T2A_puro_dTK
UsePiggybac exciseableExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)
Plasmid#62955PurposeSinglet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
Available SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Blast
Plasmid#227267PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTag-P2A-QFGal4-SV40pA
Plasmid#155127PurposeDonor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld methodDepositorInsertP2A-QFGal4
UseCRISPR and Synthetic BiologyAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsmCherryExpressionBacterialAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHDR-FOXL2-T2A-tdTomato-PuroTK
Plasmid#192892PurposeHDR donor for inserting FOXL2-T2A-tdTomato reporterDepositorInsertFOXL2-tdTomato (FOXL2 Human)
UseCRISPRMutationT2A-tdTomato fluorescent reporterPromoterPGKAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-U6-3-t-attP40
Plasmid#133561PurposegRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructsDepositorInsertattP40 region guide RNAs
UseCRISPRExpressionInsectPromoterU6Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRE57I-GNAc
Plasmid#170637PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, GNAc cassette, lacI
ExpressionBacterialAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-puroR-p2a-HA-halo
Plasmid#161979Purposehomologous donor template for endogenous taggingDepositorInsertpuroR-p2a-HA-halo-GSlinker
Available SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
mMaroon-PAK-mMaroon
Plasmid#84361PurposeFRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-PAK-mMaroon
TagsmMaroon flanking PAK domain on N and C terminusExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK382
Plasmid#214373PurposeTet-OsTIR1(F74G) (AAVS1 insertion donor)DepositorInsertTet-OsTIR1(F74G)
ExpressionMammalianPromoterTet ON 3G promoterAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1
Plasmid#184398PurposeDonor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1DepositorInsertRenilla luciferase beta-globin-PTC39
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPTC in beta-globin at amino acid position 39PromoterTet-On 3GAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1
Plasmid#184396PurposeDonor plasmid for stable integration of Renilla luciferase control reporter at AAVS1DepositorInsertRenilla luciferase beta-globin
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianPromoterTet-On 3GAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHD-mW-attPCC-FRT (pTL717)
Plasmid#115158PurposeVector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.DepositorInsertmW attP-CC FRT
ExpressionInsectAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ145
Plasmid#98405PurposeDonor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsUseCRISPR and Synthetic BiologyAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ149
Plasmid#98407PurposeDonor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsUseCRISPR and Synthetic BiologyAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-P2A-Zeo-3'
Plasmid#110408PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2-P2A-ZeoDepositorAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only