We narrowed to 11,646 results for: emb
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Plasmid#158979PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.DepositorInsertGCaMP6f-mScarlet fusion
ExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23118 (sigma 70)Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLR5-CBh-dCas9-hUtx-IRES-Hyg
Plasmid#122374PurposeExpresses dCas9-hUtx-IRES-Hyg in mammalian cellsDepositorInsertCBh-dCas9-hUtx-IRES-Hyg-pA
UsePiggybacExpressionMammalianAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-GFP-RGG
Plasmid#124948PurposeExpression of RGG-GFP-RGGDepositorAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_SBP-EGFP-WNT3A
Plasmid#108344Purposesynchronize trafficking of WNT3A from the ER (RUSH System)DepositorInsertWNT3A, Wnt family member 3A (WNT3A Human)
TagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
UBQ10:sXVE:(MCS)-S11-DI-GFP1–9
Plasmid#108260PurposeBinary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Oct4-Cr1
Plasmid#66989PurposeExpresses gRNA that targets stop codon for OCT4DepositorInsertgRNA for Crispr targeting the OCT4 stop codon
ExpressionMammalianAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SLAMF6
Plasmid#23396DepositorInsertSLAMF6 (SLAMF6 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-CMV4
Plasmid#72284PurposeExpress gene of interest under CMV promoter. WPRE has been removed to increase the cloning capacity.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterCMV promoterAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX
Plasmid#154910Purpose1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.DepositorInsertPhytochrome B (PHYB Synthetic, Mustard Weed)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYBPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP2B_LAP
Plasmid#101848PurposeTet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCHMP2B (CHMP2B Human)
UseFlp-frt recombination, tet-inducible expressionTagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationsilent mutation in Thr 104; A to TPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEAK10-His-Myc-Kal12
Plasmid#25442DepositorInsertKal12
TagsHis and MycExpressionMammalianAvailable sinceJuly 15, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME18S-3HA-mULK2
Plasmid#22898DepositorPublicationAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NanoLuc-PM-Venus
Plasmid#164786PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
TagsNanoLuc and mVenusExpressionMammalianPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2delta-DHFR
Plasmid#163760PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFRDepositorInsertb2delta-DHFR (Dhfr Mouse, Budding Yeast)
ExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046806
Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMM2-hPMCA2w/b
Plasmid#47583DepositorAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMM2-hPMCA2z/b
Plasmid#47581DepositorAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPW3758 : CMVd3-HsIRE1-HaloTag
Plasmid#185680PurposeLow-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
ExpressionMammalianAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cFos-XRI-V5
Plasmid#178058PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.DepositorInsertXRI-V5
UseAAVTagsV5-MBPExpressionMammalianPromoterMouse c-fos promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIDK-HDAC1fl
Plasmid#109158PurposeEncodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
ExpressionInsectPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only