Addgene

We narrowed to 6,446 results for: poly

Showing: 2641 - 2670 of 6446 results

Results per page:

Sort results by:

pBP-T7RNAP

Plasmid

#74096

Purpose

T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7 RNAP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
JME4472

Plasmid

#129658

Purpose

URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM4Z-EGFP

Plasmid

#183475

Purpose

This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

Depositor

Chantal Pichon

Article

Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
S-HBsAg

Plasmid

#103013

Purpose

Expresses HBV Small envelope glycoprotein in mammalian cells

Depositor

Wang-Shick Ryu

Article

Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

Insert

HBV Small envelope protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
M-HBsAg

Plasmid

#103012

Purpose

Expresses HBV Medium envelope glycoprotein in mammalian cells

Depositor

Wang-Shick Ryu

Article

Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

Insert

HBV Medium envelope protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Article

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pmNFL

Plasmid

#83127

Purpose

Mouse neurofilament protein L cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFL (Nefl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2B -1538/+64

Plasmid

#24942

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

Available since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript II SK--POLQ

Plasmid

#64873

Purpose

cloning vector for human POLQ

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-11-LECT2-I40

Plasmid

#183032

Purpose

Expression of LECT2 I40 variant protein from T7 promoter

Depositor

Gareth Morgan

Article

Belonogov et al bioRxiv 2022.03.01.482540

Insert

LECT2 (LECT2 Human)

Use

Tags

Expression

Bacterial

Mutation

I40 variant

Promoter

T7

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM4Z-Fluc

Plasmid

#183474

Purpose

This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

Depositor

Chantal Pichon

Article

Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-RRN3/TIF1A

Plasmid

#17661

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RRN3/TIF1A (RRN3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

See depositor comments below

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
ELP-TEMP/pcDNA3.1(-)

Plasmid

#178434

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

Insert

ELP-TEMP

Use

Tags

No tag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK522-FAST-PETase

Plasmid

#214754

Purpose

plasmid for expression FAST-PETase in Pseudomonas putida

Depositor

Hal Alper

Article

Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

Insert

FAST-PETase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-Pol-IIΔ

Plasmid

#35176

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the entire C-terminal domain (repeats…

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Use

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc tag WT PTB

Plasmid

#23024

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
Dazl-CPG-TV

Plasmid

#70144

Purpose

a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL

Use

Mouse Targeting and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
UtB2N7

Plasmid

#161015

Purpose

ubiquitously expressed nuclear localized tagBFP2

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

tagBFP2

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-tCorn

Plasmid

#106233

Purpose

Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LifeAct-scramble

Plasmid

#217499

Purpose

Expresses LifeAct-mScarlet-scramble to serve as control plasmid for LifeAct-MTED

Depositor

Erik Dent

Article

Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

Insert

LifeAct-mScarlet-scramble

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p3.1-NEFH

Plasmid

#26978

Depositor

David Sidransky

Article

Kim et al PLoS One. 2010 . 5(2):e9003.

Insert

NEFH (NEFH Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
LentiV_Blast

Plasmid

#111887

Purpose

Lentiviral expression of cDNA with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IVT-MCP-Phi29

Plasmid

#207456

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-Phi29

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pATetO 6XHis-Taq DNA Pol

Plasmid

#178782

Purpose

Inducible expression of Taq DNA polymerase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiV_Puro

Plasmid

#111886

Purpose

Lentiviral expression of cDNA with puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2-GCaMP3

Plasmid

#43917

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

GCaMP3

Use

Lentiviral

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

E247V in GCaMP3

Promoter

Human MAP2 promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge