We narrowed to 6,502 results for: poly

1. pGBW-m4046241

   Plasmid

   #148997

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pNIC28-BpWcbI

   Plasmid

   #125591

   Purpose

   Overexpression plasmid for production of WcbI from Burkholderia pseudomallei

   Depositor

   Nicholas Harmer

   Article

   Vivoli et al IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 1

   Insert

   BpWcbI (wcbI Burkholderia pseudomallei)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIAGO-Hap2

   Plasmid

   #114460

   Purpose

   Expression of hybrid AmphDI-PerDI (Hap) glycosyltansferase and perosamine synthase in streptomycetes

   Depositor

   Patrick Caffrey

   Article

   Hutchinson et al Chem Biol. 2010 Feb 26;17(2):174-82. doi

   Insert

   Hap2 + perDII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ermE

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTOPO-rDNA-NTS

   Plasmid

   #23992

   Depositor

   Susan Baserga

   Article

   Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

   Insert

   rDNA NTS
   

   Use

   Bacterial cloning - topo

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pGMEH-P38-luxABct6

   Plasmid

   #27063

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

   Insert

   P#38-luxAB-DAS+4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SF-1 (antisense)

   Plasmid

   #28181

   Depositor

   Bernard Schimmer

   Article

   Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

   Insert

   SF-1 (Nr5a1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Reverse Orientation

   Promoter

   Available since

   May 12, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pG6(3'Pro) (P#1478)

   Plasmid

   #14660

   Depositor

   Matija Peterlin

   Article

   Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

   Insert

   UAS repeats
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCellFree_G03 PC

   Plasmid

   #67132

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PC (PC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pJB75

   Plasmid

   #226320

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-(Y to A MR1-6)-strep
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFH77

    Plasmid

    #125816

    Purpose

    Level0 enCas9-PolI3M-TBD, wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    enCas9-PolI3M-TBD, wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PCMV-flag-Caspase11

    Plasmid

    #21145

    Depositor

    Junying Yuan

    Article

    Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

    Insert

    Caspase11 (Casp4 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pATetO 6XHis-Taq DNA Pol

    Plasmid

    #178782

    Purpose

    Inducible expression of Taq DNA polymerase in E. coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    Taq DNA Polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ELP-TEMP/pcDNA3.1(-)

    Plasmid

    #178434

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

    Insert

    ELP-TEMP
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmCherry-FKBP12-C1

    Plasmid

    #67900

    Purpose

    Expresses a fusion of mCherry with FKBP12 in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-FKBP12
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Paul Blainey, Stephen Quake

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. stathmin-GFP

    Plasmid

    #86782

    Purpose

    Mammalian expression of stathmin-GFP

    Depositor

    Lynne Cassimeris

    Article

    Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

    Insert

    stathmin (STMN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAP-BAC

    Plasmid

    #120229

    Purpose

    TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p70a-T7RNAP

    Plasmid

    #105683

    Purpose

    Expresses T7 polymerase in cell-free TXTL.

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pScPPX1

    Plasmid

    #233713

    Purpose

    Bacterial expression of the yeast PPX1 gene

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    ScPpx1 (PPX1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAV-U6+27-tCorn

    Plasmid

    #106233

    Purpose

    Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

    Insert

    tRNA-Corn
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEM4Z-Fluc

    Plasmid

    #183474

    Purpose

    This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hMSH2 Clone E3 (full-length)

    Plasmid

    #16453

    Depositor

    Bert Vogelstein

    Article

    Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

    Insert

    MSH2 (MSH2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-Pol-IIΔ

    Plasmid

    #35176

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the entire C-terminal domain (repeats…

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3 Tat HA (P#29)

    Plasmid

    #14654

    Depositor

    Matija Peterlin

    Article

    Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

    Insert

    Tat (tat HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBTK522-FAST-PETase

    Plasmid

    #214754

    Purpose

    plasmid for expression FAST-PETase in Pseudomonas putida

    Depositor

    Hal Alper

    Article

    Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

    Insert

    FAST-PETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBP-T7RNAP

    Plasmid

    #74096

    Purpose

    T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. S-HBsAg

    Plasmid

    #103013

    Purpose

    Expresses HBV Small envelope glycoprotein in mammalian cells

    Depositor

    Wang-Shick Ryu

    Article

    Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

    Insert

    HBV Small envelope protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEasy Flox

    Plasmid

    #11725

    Depositor

    Klaus Rajewsky

    Article

    Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

    Insert

    pEasyFlox
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3.1-NEFH

    Plasmid

    #26978

    Depositor

    David Sidransky

    Article

    Kim et al PLoS One. 2010 . 5(2):e9003.

    Insert

    NEFH (NEFH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only