We narrowed to 2,349 results for: 683

1. tRNA-gRNA position [2_n-1] (GB1206)

   Plasmid

   #75407

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [2_n-1]
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pADSL-2XStrep

   Plasmid

   #125684

   Purpose

   expresses human ADSL protein with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   ADSL (ADSL Human)
   

   Use

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. tRNA-gRNA position [D1_2] (GB1205)

   Plasmid

   #75406

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-miniSOG-CDC42-C-10

   Plasmid

   #55087

   Purpose

   Localization: Membrane, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   CDC42 (CDC42 Human)
   

   Use

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pEGB 35s:dCas:VP64:tNos (GB1189)

   Plasmid

   #75403

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas:VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-Basic-Fascin-Promoter (-402-0)

   Plasmid

   #89823

   Purpose

   Promoter luciferase assay

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Fascin1 (FSCN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PRKD3 gRNA (BRDN0001146689)

   Plasmid

   #75951

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKD3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKD3 (PRKD3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PRKD3 gRNA (BRDN0001146945)

   Plasmid

   #75953

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKD3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKD3 (PRKD3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Luc-Hdm4-3'UTR-F2d5

   Plasmid

   #64018

   Purpose

   Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5

   Depositor

   Lin He

   Article

   Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

   Insert

   HDMX (MDM4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_MIA3-1/1

    Plasmid

    #91327

    Purpose

    Protein expression and purification of human SH3 domain construct MIA3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MIA3-1/1 (MIA3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3b(1454/3172)P2P-mut

    Plasmid

    #21405

    Depositor

    Eric Metzen

    Article

    Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    hypoxia response element mutated

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)

    Plasmid

    #51390

    Depositor

    Narry Kim

    Article

    Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

    Insert

    NM_001039802.1 (CDC42 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    miR-29 binding site mutant (position 577-599, 997…

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pOP795

    Plasmid

    #235700

    Purpose

    Protein expression His-taged HsNRBF2_FL in bacteria

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    NRBF2 (NRBF2 Human)
    

    Use

    Tags

    His6(TEV)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOP796

    Plasmid

    #235701

    Purpose

    Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    NRBF2 MIT domain (NRBF2 Human)
    

    Use

    Tags

    His6(TEV)

    Expression

    Bacterial

    Mutation

    aa 1-89

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR1A 2xHA GPR137

    Plasmid

    #226809

    Purpose

    Gateway entry vector for GPR137

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR137 (GPR137 Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_001164351

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-CAPN7-OSF

    Plasmid

    #193041

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPICZ A-SLC-His+Strep-Opa1 (SB254)

    Plasmid

    #227603

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    Opa1
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 253-960 only

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

    Plasmid

    #227606

    Purpose

    Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    R247A,R347A

    Promoter

    AOX1

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

    Plasmid

    #227620

    Purpose

    Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R257A,R347A

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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