Addgene

We narrowed to 4,087 results for: Erf

Showing: 1761 - 1780 of 4087 results

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pMDC32B-B/c

Plasmid

#227963

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v10 mammalian expression

Plasmid

#208589

Purpose

Expression of SynCas.v10 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

SynCas.v10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v14A mammalian expression

Plasmid

#208590

Purpose

Expression of SynCas.v14A for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

SynCas.v14A

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v35 mammalian expression

Plasmid

#208591

Purpose

Expression of SynCas.v35 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

SynCas.v35

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v39 mammalian expression

Plasmid

#208592

Purpose

Expression of SynCas.v39 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

SynCas.v39

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v41 mammalian expression

Plasmid

#208593

Purpose

Expression of SynCas.v41 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

SynCas.v41

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-SynCas.v10 bacteria

Plasmid

#208594

Purpose

Production and purification of catalytically active SynCas.v10

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

SynCas.v10

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria

Plasmid

#208595

Purpose

Production and purification of catalytically inactive SynCas.v10

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

dSynCas.v10

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-SynCas.v12 bacteria

Plasmid

#208596

Purpose

Production and purification of catalytically active SynCas.v12

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

SynCas.v12

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria

Plasmid

#208597

Purpose

Production and purification of catalytically inactive SynCas.v12

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

dSynCas.v12

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v47 mammalian expression

Plasmid

#209003

Purpose

Expression of SynCas.v47 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

SynCas.v47

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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tdiRFP-BFP reporter

Plasmid

#190184

Purpose

The cre-activatable fluorescence reporter

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

tdiRFP
TagBFP2

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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FAM177A1-Halo

Plasmid

#224591

Purpose

For expression of FAM177A1-Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Use

Tags

HALO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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ZFPL1-GFP

Plasmid

#224590

Purpose

For expression of ZFPL1-GFP in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

ZFPL1 (ZFPL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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FAM177A1-SNAP

Plasmid

#224592

Purpose

For expression of FAM177A1-SNAPTag in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Use

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28-Strep-OPA1(253-960) (SB263)

Plasmid

#227612

Purpose

Inducible expression of Strep-tagged Opa1(253-960) in E.coli

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Use

Tags

Twin-StrepTag

Expression

Bacterial

Mutation

contains aa 253-960 only

Promoter

T7

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28-Strep-OPA1(MGD) (SB264)

Plasmid

#227613

Purpose

Inducible expression of Strep-tagged Opa1(MGD) in E.coli

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Use

Tags

Twin-StrepTag

Expression

Bacterial

Mutation

contains aa 253-580 (MGD) and aa 938-960

Promoter

T7

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46-His (SB250)

Plasmid

#227599

Purpose

Inducible expression of His-tagged SLC25A46 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Use

Tags

10xHis

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1(MGD) (SB252)

Plasmid

#227601

Purpose

Inducible expression of Strep-tagged Opa1(MGD) in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Use

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-580 (MGD) and aa 938-960

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)

Plasmid

#227614

Purpose

Overexpression of SLC25A46 in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Use

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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