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LENTICRISPR-HK2_sgRNA1

Plasmid

#201596

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGria2

Plasmid

#124868

Purpose

Mutagenesis of Gria2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria2 (Gria2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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human ETV6 gRNA-1

Plasmid

#133353

Purpose

human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ACTB_sgRNA

Plasmid

#183885

Purpose

pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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Dom neg CstF64

Plasmid

#127250

Purpose

Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

CstF64 delta 282 (CSTF2 Human)

Use

Other

Tags

Expression

Mammalian

Mutation

insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

Promoter

pEF1a

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.5 shETV4-B

Plasmid

#74976

Purpose

shETV4 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

Plasmid

#118845

Purpose

Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

ptena and ptenb (ptena, ptenb Zebrafish)

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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plenti-px330-CRBN-T2-pGK-Pur

Plasmid

#107383

Purpose

Mammalian expression CRISPR/Cas9

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

sgRNA targeting CRBN (CRBN Human)

Use

Lentiviral; Crispr

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-MGAT1-KO

Plasmid

#80009

Purpose

gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

MGAT1 (MGAT1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAX-2

Plasmid

#210130

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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gh139

Plasmid

#106809

Purpose

expression of gRNA targeting EXTL3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

EXTL3 (EXTL3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin1

Plasmid

#124865

Purpose

Mutagenesis of Grin1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin1 (Grin1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgATXN1L-2

Plasmid

#74963

Purpose

Cas9 + sgATXN1L-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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PRKAG2 gRNA (BRDN0001147689)

Plasmid

#77461

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAG2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAG2 (PRKAG2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-AGO2 ts2

Plasmid

#115854

Purpose

AGO2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO2 shRNA (AGO2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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RIPK1 gRNA (BRDN0001148444)

Plasmid

#76533

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK1 (RIPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK1 gRNA (BRDN0001149461)

Plasmid

#76532

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK1 (RIPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLCHKO_GFP_Luciferase

Plasmid

#155079

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

Depositor

Jason Moffat, Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCOQ2

Plasmid

#186025

Purpose

knock out COQ2 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Coenzyme Q2, Polyprenyltransferase (COQ2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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