We narrowed to 2,075 results for: PAM

1. CyPet-FKBP-E2

   Plasmid

   #175244

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK526

   Plasmid

   #155387

   Purpose

   PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   PphlF-TA4 in dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-His:FRB:nls:VPR

   Plasmid

   #135987

   Purpose

   Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   FRB domain fused to the VPR activation domain
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. EW392 CMV-TO-NZF-(GGS)x8-FRB(T2098L) (FLP-IN)

   Plasmid

   #236157

   Purpose

   Expresses NZF transcriptional activation domain attached by a GS linker to the mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   NZF-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T2098L in FRB

   Promoter

   CMV promoter with two TetR binding sites

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-FKBP-LBR

   Plasmid

   #222427

   Purpose

   To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al bioRxiv 2024.05.31.596797

   Insert

   LBR (LBR Human)
   

   Use

   Tags

   GFP-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXD70LacZ7-DadR/DadS-Pdadh-LacZ

   Plasmid

   #191622

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadS

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2Aneo-mRFP-FKBP-mSos1-linkercat

   Plasmid

   #173858

   Purpose

   Encoding a part of the rapamycin-induced Ras activation system.

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   mRFP-FKBP-mSos1-linkercat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK528

   Plasmid

   #155388

   Purpose

   dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK434

   Plasmid

   #155378

   Purpose

   PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   PphlF_A4NT in dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK422

    Plasmid

    #155373

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK423

    Plasmid

    #155374

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK3_018

    Plasmid

    #123733

    Purpose

    Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    spdCas9(1-204)-frb-D10A-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcUW51-CgE

    Plasmid

    #13762

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

    Insert

    HSV-1 glycoprotein E
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    C-terminal region of the HSV-1 gE (KOS strain ect…

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pJK520

    Plasmid

    #155386

    Purpose

    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLPT234

    Plasmid

    #127855

    Purpose

    Repressilator 2.0, without degradation tags, integrated triple reporter

    Depositor

    Johan Paulsson

    Article

    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

    Inserts

    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    First 11aa derived from mCherry as fusion protein

    Promoter

    pAmpR, pL LacO1, pL tetO1, and pR

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IZ-SEC61B-mCherry-FRB

    Plasmid

    #233349

    Purpose

    Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    SEC61B (SEC61B Human)
    mCherry
    FRB
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T2098L mutant of FKBP-rapamycin binding domain of…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP-CD28TMD-NTEVp(H75E)

    Plasmid

    #137829

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75E)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid

    #137830

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Leucine 71 to Lysine in CTEVp fragment

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-Lck-FRB-mTurquoise2

    Plasmid

    #176130

    Purpose

    Plasma membrane localized FRB, rapamycin heterodimerization

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    FRB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST_DSUP_RAMVA (pBS0353)

    Plasmid

    #185189

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge