We narrowed to 2,847 results for: PHI-1
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Plasmid#149545PurposeExpression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMUH_unc84_2XGFP
Plasmid#46023DepositorInsertUNC-84 (unc-84 )
Use10xuas-phi31-expression vectorTags2XGFPExpressionMutationTwo amino acid mutations (F279L and L500P) compar…Promoter10XUASAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-GMR-eya(shRNA)
Plasmid#157991PurposepBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMUH_unc84_tdTFlag
Plasmid#46024DepositorInsertUNC-84 (unc-84 )
Use10xuas-phi31-expression vectorTagstdTomatoExpressionMutationTwo amino acid mutations (F279L and L500P) compar…Promoter10XUASAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_dTK
Plasmid#133244PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertdrosophila tachykinin-fused codon-optimized GCaMP6s
UseTagsExpressionInsectMutationPromoterhsp70Available sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWH-Rluc-let-7-A114-N40-HhR
Plasmid#50555PurposeExpression of luciferase reporter mRNA in S2 cellDepositorInsertLuciferase-let7-A114-N40-HhR
UseLuciferaseTagsExpressionInsectMutationPromoterActin5CAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAWH-Rluc-let-7-Poly(A)
Plasmid#50556PurposeExpression of luciferase reporter mRNA in S2 cellDepositorInsertLuciferase-let7-Poly(A)
UseLuciferaseTagsExpressionInsectMutationPromoterActin5CAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat
Plasmid#25029DepositorInsertHPat (Patr-1 Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1147-1386-3xL-Amut_P
Plasmid#147167PurposeInsect Expression of DmNot1_1147-1386-3xL-AmutDepositorInsertDmNot1_1147-1386-3xL-Amut (Not1 Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-term png in pGEX4
Plasmid#112975PurposeFor protein expression and purification of the C-term of Drosophila pngDepositorInsertpng (png Fly)
UseTagsExpressionBacterialMutationwild-type C terminusPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mMib1-FLAG
Plasmid#37116DepositorInsertMib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)
UseTags3 x FLAGExpressionMammalianMutationACC Kozak sequence added before ATG annotated.PromoterCMVAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJP118
Plasmid#176494PurposeExpresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertLifeact peptide
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT2A
Plasmid#53109PurposeExpression of Drosophila 5-HT2ADepositorInsert5HT2A (5-HT2 Fly)
UseTagsExpressionMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
C2 png genomic in Casper
Plasmid#112971PurposeP element vector for transposon insertion of png genomic DNA into Drosophila genomeDepositorInsertpng genomic DNA (png Fly)
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
C2 M2 png-myc in Casper4
Plasmid#112968PurposeP element vector for transposon insertion of Myc-tagged png into Drosophila genomeDepositorInsertpng-myc (png Fly)
UseP insertion vectorTagsmyc epitopeExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2iU6Apowriter2N(#355)
Plasmid#184106Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesisDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesis + in vitro trans…Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-Ubi+SP6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
opto-E-cad GFP
Plasmid#203327PurposeExpresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cellsDepositorInsertPhotoswitchable - E-cadherin with GFP tag (CDH1 Human)
UseTagsGFPExpressionMammalianMutationAddition of Aslov2 domain at T133 of human E-cadh…PromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNL2119 pARF7 GG0-GG1
Plasmid#195910PurposeLevel 0 pARF7 promoter with GG0 and GG1 golden gate linkersDepositorInsertpARF7 (NPH4 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only