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pHAGE2-TetO-Salhel-II-Sox2HMG

Plasmid

#231534

Purpose

Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG flanked with N- and C-termini of Salhel-II

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Salhel-I

Plasmid

#231513

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salhel-POU

Plasmid

#231522

Purpose

Expresses Salpingoeca helianthica full-length POU in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Salhel-Sox-II

Plasmid

#231516

Purpose

Expresses Salpingoeca helianthica full-length Sox-II in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-II

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Myflu-Sox-II

Plasmid

#231518

Purpose

Expresses Mylnosiga fluctuans full-length Sox-II in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-II

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi

Plasmid

#231514

Purpose

Expresses Pigoraptor chileana chimeric Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Myflu-Sox-I

Plasmid

#231517

Purpose

Expresses Mylnosiga fluctuans full-length Sox-I in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-I

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX2-C17

Plasmid

#206385

Purpose

Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX2-C17 chimera (SOX2, SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-GFP-mSox17FNV

Plasmid

#206372

Purpose

Expresses EGFP fused mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Lentiviral

Tags

EGFP

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

Plasmid

#199357

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-TERT K78E-IRES-Puro

Plasmid

#182591

Purpose

expresses TERT K78E in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Mutation

K78E

Promoter

EF-1a

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Expression

Bacterial

Mutation

lexA G85D

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_PrecA:mNeonGreen

Plasmid

#225192

Purpose

Used for inserting PrecA upstream of mNeonGreen at the bacterial Tn7 site for use as a RecA reporter

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

PrecA:mNeonGreen

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-α-Synuclein-c_myc

Plasmid

#225222

Purpose

Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

α-Synuclein (SNCA Human)
Aga2 (AGA2 Budding Yeast)

Tags

GS linker, HA Tag, and c-myc Tag

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Pseudoalteromonas_sp._A2

Plasmid

#224401

Purpose

For expression of the type I CBASS system encoded by Pseudoalteromonas sp. A2

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Psp.CdnB_AGS-C_Cap15

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Alteromonas_sp._ALT199

Plasmid

#224399

Purpose

For expression of the type I CBASS system encoded by Alteromonas sp. ALT199

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Asp.CdnB_AGS-C_Cap15

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pWP001

Plasmid

#178596

Purpose

pAF256 derived plasmid encoding a xylose inducible CD16/50L CBD-SmBiT and LgBiT

Depositor

Robert Fagan

Article

Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

Insert

CBD-SmBiT/LgBiT

Expression

Bacterial

Promoter

Pxyl

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)

Plasmid

#160583

Purpose

Part 1/3 for assembly of site-specific recombination based Registers. Composed of the reversed sequences of the TNos terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

YFP

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD 5'UTR:YFP:Tnos (GB1483)

Plasmid

#160557

Purpose

Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

5'UTR:YFP:Tnos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET16b-Rnl2(1-249)K227Q

Plasmid

#14072

Depositor

Thomas Tuschl

Insert

Rnl2(1-249)

Tags

His (10x)

Expression

Bacterial

Mutation

changed Lys227 to Glutamine Gly112 is actually C…

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only