168,368 results
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Plasmid#173044PurposeTnC-based calcium sensor with green-red FRET pair ratiometric readoutDepositorHas ServiceAAV2InsertNES-Twitch-GR
UseAAVExpressionMammalianPromoterCamK2Available SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-GA
Plasmid#209871PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddGFP A
TagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Src-Y530F
Plasmid#124659PurposeHuman c-Src with Y530F mutationDepositorAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-N7HELIX-KanR
Plasmid#190663PurposeKanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequenceDepositorInsertKanR, R6K origin of replication
ExpressionBacterialPromoterNAAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK344 (Hygro-P2A-mAID)
Plasmid#121178PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEND-320_Cacnes_ori
Plasmid#225602PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
NFAT/AP-1 3x luciferase
Plasmid#11783DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-SPI1
Plasmid#162824PurposeDoxycycline-inducible overexpression of human SPI1DepositorAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEM-GECO
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable SinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only