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Plasmid#195278PurposeAllows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE-P48R-UGI
Plasmid#161817PurposeExpresses ABE-P48R-UGI in mammalian cellsDepositorInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpdCas9-VP64
Plasmid#115794PurposeExpresses SpdCas9-Vp64 fusion protein when packaged into AAVDepositorInsertVP64
UseAAVTags3XFLAG and dCas9-VP64ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC591 - pCMV Mem-iRFP
Plasmid#59134PurposeA plasmid that express membrane-targeted iRFP under the CMV promoter.DepositorInsertMembrane-localized Infrared Fluorescent Protein
UseTagsPalmitylation signalExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOX13-Tier1-PhCMV-tGFP
Plasmid#169510PurposeTier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).DepositorInsertPCMV-driven turbo green fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFOX12-Tier1-PhCMV-eGFP
Plasmid#169509PurposeTier-1 vector encoding PhCMV-driven green fluorescent protein eGFP (PhCMV-eGFP-pA).DepositorInsertPCMV-driven enhanced green fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA-CMV-GFP
Plasmid#85451PurposeExpress sgRNA in mammalian cellsDepositorInsertpCMV-EGFP
UseAAVTagsExpressionMutationPromoterpCMV-EGFPAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
NoLS-Grx1-roGFP2
Plasmid#236545PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleoli.DepositorInsertNoLS-Grx1-roGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT-short-UBASH3A-GFP11
Plasmid#195385PurposeMammalian expression of human wild-type short-form UBASH3A with GFP(11) tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsGFP(11)ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2B-sfGFP-T2A-iCre
Plasmid#178546PurposepCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRTDepositorInsertHistone H2B-sfGFP-T2A-iCre
UseCre/Lox and Mouse Targeting; Flp/frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
NES-Grx1-roGFP2
Plasmid#236551PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.DepositorInsertNES-Grx1-roGFP2 (NES Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
H2B-Grx1-roGFP2
Plasmid#236550PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleus.DepositorInsertH2B-Grx1-roGFP2 (H2BC21 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
G3BP1-Grx1-roGFP2
Plasmid#236548PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in stress granules.DepositorInsertG3BP1-Grx1-roGFP2 (G3BP1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
Grx1-roGFP2-Dcp1b
Plasmid#236547PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in processing bodies.DepositorInsertGrx1-roGFP2-Dcp1b (DCP1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
Grx1-roGFP2-Coilin
Plasmid#236546PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in Cajal bodies.DepositorInsertGrx1-roGFP2-Coilin (COIL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1)
Plasmid#233273PurposeTo express GFP from a CMV promoter and to express and shRNA targeting Rat Nurr1DepositorInsertGFP and shRNA targeting Rat NPAS1 (Npas1 Rat)
UseAAVTagsGFPExpressionMutationPromoterAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #5)
Plasmid#233274PurposeTo express GFP from a CMV promoter and to express and shRNA targeting Rat Nurr1DepositorInsertGFP and shRNA targeting Rat NPAS1 (Npas1 Rat)
UseAAVTagsGFPExpressionMutationPromoterAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
PNCA-GFP
Plasmid#67789PurposeUse together with pCMV-intron and VSV-G to package MMLV reporter virusDepositorInsertMMLV-LTR-GFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
PNCA-GFP-dYY1
Plasmid#67791PurposeUse together with pCMV-intron and VSV-G to package MMLV reporter virusDepositorInsertMMLV-LTR-GFP
UseTagsExpressionMutationDeletion of LTR U3 YY1 binding domainPromoterAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only