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Human HMG-CoA lyase (HL)

Plasmid

#52433

Purpose

bacterial expression of human HMG-CoA lyase (HL)

Depositor

Henry Miziorko

Article

Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

Insert

HMG CoA lyase (HMGCL Human)

Use

Tags

His and S

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
CS51

Plasmid

#117433

Purpose

Expression of ARL3

Depositor

Richard Kahn

Article

Cavenagh et al J Biol Chem. 1994 Jul 22;269(29):18937-4

Insert

ARL3 (ARL3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pJS278

Plasmid

#134139

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255, R155A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hPCNA-C148S

Plasmid

#190939

Purpose

untagged human PCNA with a cysteine to serine mutation

Depositor

Brian Kelch

Article

Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Use

Tags

Expression

Bacterial

Mutation

changed cysteine 148 to serine

Promoter

T7

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDK616

Plasmid

#134202

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

K223A K226A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pIW040

Plasmid

#134127

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 R236A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW039

Plasmid

#134126

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 and K203A K206A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW028

Plasmid

#134125

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 and K183A K184A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#117429

Purpose

Expression of ARL1

Depositor

Richard Kahn

Article

Van Valkenburgh et al J Biol Chem. 2001 Jun 22;276(25):22826-3

Insert

ARL1 (ARL1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL15

Plasmid

#117430

Purpose

Expression of ARL2

Depositor

Richard Kahn

Article

Clark et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

ARL2 (ARL2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Cb2

Plasmid

#53529

Purpose

Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli

Depositor

Gabor Igloi

Article

Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)

Insert

mitochondrial arginyl-tRNA synthetase

Use

Tags

His Tag and Thioredoxin

Expression

Bacterial

Mutation

changed Ser4 to Ala4 to permit NcoI cloning

Promoter

T7

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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HsCLYBL(aa23-340)-His

Plasmid

#111288

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL ( aa23-340) codon optimized for bacterial expression (CLYBL Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pDK637

Plasmid

#134203

Purpose

bacterial expression of human CENP-N 1-240

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N 1-240-His (CENPN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HsCLYBL(aa23-340) D320N-His

Plasmid

#111289

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL ( aa23-340) D320N codon optimized for bacterial expression (CLYBL Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM30-ARC105

Plasmid

#133426

Purpose

Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Use

Tags

Expression

Bacterial

Mutation

Contains amino acids 5-78 fused to C-terminal of …

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTK41

Plasmid

#236110

Purpose

Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody

Depositor

Shinsuke Niwa

Article

Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb

Inserts

KIF5C(C7S)(1-430)-SNAP-His (Kif5c Rat)
KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag (Kif5c Rat)

Use

Tags

ALFA tag nanobody, His tag, SNAP tag, and StrepII…

Expression

Bacterial

Mutation

C7S mutation is introduced. 1-430 aa is encoded.

Promoter

T7

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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RALA V25L

Plasmid

#122927

Purpose

Bacterial expression of RALA V25L

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

V25L

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA V25M

Plasmid

#122928

Purpose

Bacterial expression of RALA V25M

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

V25M

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA D130G

Plasmid

#122929

Purpose

Bacterial expression of RALA D130G

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

D130G

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA S157A

Plasmid

#122930

Purpose

Bacterial expression of RALA S157A

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

S157A

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA R176X

Plasmid

#122931

Purpose

Bacterial expression of RALA R176X

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

R176X

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA WT

Plasmid

#122925

Purpose

Bacterial expression of RALA WT

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA G23D

Plasmid

#122926

Purpose

Bacterial expression of RALA G23D

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

G23D

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23 C3_13

Plasmid

#60298

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

EDN3 enhancer (EDN3 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_13

Plasmid

#60261

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

EDN3 enhancer (EDN3 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge