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PB-Antares2

Plasmid

#120868

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-Antares2

Use

Tags

Expression

Mammalian

Mutation

Antares2 amplicon

Promoter

CAG Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVenus_Lys-less-Cdc20 (1620)

Plasmid

#39849

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Lys-less = all Lys converted to Arg

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
Venus-Cdc20 R132A (1768)

Plasmid

#26059

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Mutation in the Mad2 binding site, R132A

Promoter

Available since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only
pLPT234

Plasmid

#127855

Purpose

Repressilator 2.0, without degradation tags, integrated triple reporter

Depositor

Johan Paulsson

Article

Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

Inserts

bla
mSCFP3
mKate2
mVenus
TetR
LacI
cI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

First 11aa derived from mCherry as fusion protein

Promoter

pAmpR, pL LacO1, pL tetO1, and pR

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-tdTomato

Plasmid

#97079

Purpose

Retroviral plasmid containing tdTomato for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVII-LO

Plasmid

#162754

Purpose

Retroviral (MSCV) expression of LSSmOrange fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

LSSmOrange

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJK504

Plasmid

#155381

Purpose

dCas12a crRNAs

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

dCas12a oscillator crRNAs

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNb_opt.IRES.eGFP

Plasmid

#22492

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

Codon optimised wild type Calcineurin B (PPP3R1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pLTR-G

Plasmid

#17532

Depositor

Jakob Reiser

Article

Reiser et al Proc Natl Acad Sci U S A. 1996 Dec 24. 9

Insert

VSV-G glycoprotein (Indiana strain)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mIFP

Plasmid

#96951

Purpose

Retroviral plasmid containing mIFP for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Infrared Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Venus-Flag (865)

Plasmid

#40998

Depositor

Jonathon Pines

Article

Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

Type

Empty backbone

Use

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-Sapphire

Plasmid

#96950

Purpose

Retroviral plasmid containing Sapphire for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Sapphire fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Venus

Plasmid

#96940

Purpose

Retroviral plasmid containing Venus for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Venus Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mApple

Plasmid

#96934

Purpose

Retroviral plasmid containing mApple for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Apple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mCerulean3

Plasmid

#96936

Purpose

Retroviral plasmid containing mCerulean3 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Cerulean 3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mOrange2

Plasmid

#96938

Purpose

Retroviral plasmid containing mOrange2 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Orange2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJK398

Plasmid

#155370

Purpose

dCas9 sgRNA oscillator v1 (dCRv1) (p15A)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pJK517

Plasmid

#155385

Purpose

PphlF-TA4 in crRNAs

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

PphlF-TA4 in dCas12a oscillator crRNAs

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_StrepII_TEV_PP2Ac

Plasmid

#217477

Purpose

Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

Depositor

Wolfgang Peti

Article

Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

Insert

PP2Ac

Use

Tags

StrepII, TEV

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Tags

Expression

Mutation

Codon Optimised wtCNa

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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