We narrowed to 4,833 results for: phy

1. AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC

   Plasmid

   #125695

   Purpose

   Global labeling of inhibitory synapses (red fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   mScarlet-Gephyrin.FingR-IL2RGTC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR SFFVp Phy-mCherry-CAAX

   Plasmid

   #50839

   Purpose

   Optogenetic PI3K activation system; membrane component

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PhyB 1-908
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. GC1 pComb3xss anti-Gephyrin nanobody

   Plasmid

   #134720

   Purpose

   Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Gephyrin (Human) recombinant llama nanobody GC1 (GPHN L. glama (llama))
   

   Use

   Tags

   6xHis-HA

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #125692

   Purpose

   Global labeling of inhibitory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GC52 pEGFPN1 anti-Gephyrin nanobody

   Plasmid

   #135224

   Purpose

   Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

   Plasmid

   #105715

   Purpose

   In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

   Depositor

   Rylan Larsen

   Has Service

   AAV5

   Insert

   synaptophysin-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GC83 pComb3xss anti-Gephyrin nanobody

   Plasmid

   #134721

   Purpose

   Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Gephyrin (Human) recombinant llama nanobody GC83 (GPHN L. glama (llama))
   

   Use

   Tags

   6xHis-HA

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHybr_r2a>m2a(silent)-srt-his

   Plasmid

   #124807

   Purpose

   HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.

   Depositor

   Martijn Verdoes, Ferenc Scheeren

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG2a (Fc silent)
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Mutation

   L234A/L235A/N297A

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GC92 pComb3xss anti-Gephyrin nanobody

   Plasmid

   #145826

   Purpose

   Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Gephyrin (Human) recombinant llama nanobody GC92 (GPHN L. glama (llama))
   

   Use

   Tags

   6xHis-HA

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GS8 pComb3xss anti-Gephyrin nanobody

    Plasmid

    #145827

    Purpose

    Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Gephyrin (Human) recombinant llama nanobody GS8 (GPHN L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GS41 pComb3xss anti-Gephyrin nanobody

    Plasmid

    #145828

    Purpose

    Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Gephyrin (Human) recombinant llama nanobody GS41 (GPHN L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

    Plasmid

    #139482

    Purpose

    Expresses PhyB621-mCherry-HRasCT in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    PhyB621 (PHYB Mustard Weed)
    

    Use

    Lentiviral

    Tags

    mCherry-HRasCT

    Expression

    Mammalian

    Mutation

    G229L and deletion of aa Y at position 235 in mch…

    Promoter

    Tet responsible element

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHyg-CUbo(K48R/K63R)-His6

    Plasmid

    #212780

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHyg-CUbo(K48R/K63R)-yeGFP

    Plasmid

    #212769

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHyg-CUbo(K48R)-yeGFP-His6

    Plasmid

    #212783

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHyg-CUbo(K63R)-yeGFP-His6

    Plasmid

    #212784

    Purpose

    C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHyg-CUbo(K48R/K63R)-9myc

    Plasmid

    #212789

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hPhyB621(Y276H)-P2A-m1V

    Plasmid

    #197377

    Purpose

    The AAV vector for PhyB621(Y276H) and m1V

    Depositor

    Michiyuki Matsuda

    Insert

    Phytochrome B Y276H(1-621 aa)
    

    Use

    AAV

    Tags

    P2A-m1V

    Expression

    Mutation

    Human codon optimized PhyB (1-621 aa), Y276H muta…

    Promoter

    CMV

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #126217

    Purpose

    Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge