We narrowed to 610 results for: prime editing

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pGEL686

Plasmid

#207510

Purpose

ePPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Insert

zCas9 (H840A)-NC-M-MLV RT-deltaRH

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLSL.R.Ly

Plasmid

#227697

Purpose

Reengineered geminiviral replicon vector

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-PEmax-P2A-Puro

Plasmid

#228890

Purpose

Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Inserts

PEmax
Puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-AAVS1_B3903c_attP

Plasmid

#182144

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

AAVS1_B3903c_attP pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-AAVS1_A3786c_attP

Plasmid

#182143

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

A3786c-attP pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAC026

Plasmid

#227598

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

Depositor

Brittany Adamson

Article

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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attB-puro-GA donor

Plasmid

#182138

Purpose

attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-GA-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_PEmax-2A-BSD

Plasmid

#234071

Purpose

For lentiviral expression of PEmax with blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-CCR5_DF_B584b_attB

Plasmid

#182148

Purpose

To insert attB attachment site at CCR5 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

CCR5-B584b-attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-CCR5_DF_A509b_attB

Plasmid

#182147

Purpose

To insert attB attachment site at CCR5 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

CCR5-A509b-attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-peg

Plasmid

#141081

Purpose

Gateway compatible prime editing gRNA empty backbone vector

Depositor

Yiping Qi

Article

Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET30a(+)-PE2

Plasmid

#180015

Purpose

Expressing PE2 protein in E.coli for protein purification

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2

Tags

6xHis

Expression

Bacterial

Promoter

Lac operator-controlled T7 promoter

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHSG1C3

Plasmid

#164423

Purpose

single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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attP-puro-GA donor

Plasmid

#182140

Purpose

attP-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_B358c_attB

Plasmid

#182146

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

ALB_B358c_attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_A277c_attB

Plasmid

#182145

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

Alb_A277c_attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

Plasmid

#182151

Purpose

To insert attB-rev attachment site at IDS in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS-C2c-attBrev pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-hP53DD

Plasmid

#214084

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PEmax-P2A-hP53DD (TP53 SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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