Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,302 results for: BLI;

Clear keywords
Showing: 4451 - 4500 of 9302 results

  2. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Expression
    Bacterial
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available Since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

    Enlarge

  3. pBABEpuro-CRAF_S259A

    Plasmid
    #51127
    Purpose
    mammalian expression of wt CRAF S259A
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    S259A
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51127-plasmid-map-sequence-id-77385

    Enlarge

  4. pINDUCER20-hMYF5-3xHA (#732)

    Plasmid
    #78332
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    MYF5 (MYF5 Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Promoter
    TRE2
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. PTPRN2

    Plasmid
    #38891
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRN2 (PTPRN2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)

    Plasmid
    #44089
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44089-plasmid-map-sequence-id-61674

    Enlarge

  8. 2P0E

    Plasmid
    #60109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. MIGR1-Meis1a

    Plasmid
    #78148
    Purpose
    retroviral expression of Meis1a and GFP
    Depositor
    Francesco Blasi
    Insert
    Meis1a (Meis1 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Available Since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. RPS6KA1

    Plasmid
    #39021
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RPS6KA1 (RPS6KA1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid
    #44072
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44072-plasmid-map-sequence-id-61692

    Enlarge

  14. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dTET1
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104413-plasmid-map-sequence-id-204141

    Enlarge

  15. SETD3

    Plasmid
    #32873
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD3 (SETD3 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. PRDM11

    Plasmid
    #32858
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM11 (PRDM11 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 79-314
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53206
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and TRP1
    Promoter
    GAL1 promoter
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. HaloTag-G protein gamma-7

    Plasmid
    #67040
    Purpose
    G protein gamma-7 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-7 (GNG7 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67040-plasmid-map-sequence-id-115582

    Enlarge

  19. pFR022

    Plasmid
    #134228
    Purpose
    bacterial expression of KNL1
    Depositor
    Iain Cheeseman
    Insert
    hKNL1 aa 2106-2316 (KNL1 Human)
    Expression
    Bacterial
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    134228-plasmid-map-sequence-id-271855

    Enlarge

  20. p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP

    Plasmid
    #185699
    Purpose
    Retroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter
    Depositor
    Lynette Lim
    Inserts
    Tdtomato,
    Synaptophysin-EGFP
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP and T2A
    Expression
    Mammalian
    Promoter
    mDlx
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185699-plasmid-map-sequence-id-366333

    Enlarge

  21. pCB32

    Plasmid
    #111911
    Purpose
    Expresses gRNA to target Nte1 locus and has HygR marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting Nte1 locus
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    SNR52p
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111911-plasmid-map-sequence-id-218313

    Enlarge

  22. pKG210

    Plasmid
    #69761
    Purpose
    Retroviral expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pLP-mOlig2-ires-gfp

    Plasmid
    #49399
    Purpose
    mammalian bicistronic expression of mouse Olig2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Olig2 (Olig2 Mouse)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP and IRES
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    49399-plasmid-map-sequence-id-316898

    Enlarge

  24. pKG186

    Plasmid
    #63076
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FANC-A, IMAGE clone 5725803 (FANCA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Available Since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    63076-plasmid-map-sequence-id-104390

    Enlarge

  25. pBABEpuro-CRAF_S257L

    Plasmid
    #51125
    Purpose
    mammalian expression of wt CRAF S257L
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    S257L
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51125-plasmid-map-sequence-id-77383

    Enlarge

  26. pAAV asyn S129E

    Plasmid
    #36068
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S129E (SNCA Human)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    S129E
    Promoter
    CMV
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  27. MLYCD

    Plasmid
    #39081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MLYCD (MLYCD Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Surface mutant for crystallisation E19A, K20A, E2…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 9 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

    Enlarge

  29. PA-TagRFP-Alpha-5-Integrin-12

    Plasmid
    #62791
    Purpose
    Localization: Focal Adhesions, Excitation:N/A, Emission:N/A
    Depositor
    Michael Davidson
    Insert
    Alpha5Integrin (ITGAV Human)
    Tags
    TagRFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid
    #104418
    Purpose
    transient expression of dCas9-dJMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    dJMJD2A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    S288G-T289G
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104418-plasmid-map-sequence-id-204359

    Enlarge

  31. ADH4

    Plasmid
    #38910
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADH4 (ADH4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. PBC002-A04

    Plasmid
    #58761
    Purpose
    bacterial expression of PDB 4PWY
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PWY (PEX6 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. FN 227

    Plasmid
    #50499
    Depositor
    Kenneth Yamada
    Insert
    FN1 fibronectin 1 (FN1 Human)
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    repeats 6-10; D1526E and R1524K
    Promoter
    T7
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. pFLAG-6a-Ataxin3Q22-C14A

    Plasmid
    #22127
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    C14A
    Available Since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only

  35. pZC11 (pFA6a-TEV-6xGly-V5-KanMX)

    Plasmid
    #44097
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available Since
    April 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    44097-plasmid-map-sequence-id-61694

    Enlarge

  36. UXS1

    Plasmid
    #39162
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    UXS1 (UXS1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. pCB100

    Plasmid
    #69746
    Purpose
    Retroviral expression of Centrin 3
    Depositor
    Iain Cheeseman
    Insert
    Centrin 3 (CETN3 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Available Since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  38. SPSB1

    Plasmid
    #38981
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPSB1 (SPSB1 Human)
    Tags
    6xHis-Tev
    Expression
    Bacterial
    Mutation
    Residues 24-233 of SPSB1 are present in this plas…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38981-plasmid-map-sequence-id-199971

    Enlarge

  39. pE-SUMO CRYBB3

    Plasmid
    #80750
    Purpose
    Expression of human beta B3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB3 (CRYBB3 Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80750-plasmid-map-sequence-id-152180

    Enlarge

  40. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Promoter
    Ef1-alpha
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

    Enlarge

  42. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180151
    Purpose
    td-sfGFP cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180151-plasmid-map-sequence-id-355316

    Enlarge

  43. mEmerald-Enconsin-N-18

    Plasmid
    #62754
    Purpose
    Localization: Microtubule, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    Enconsin (MAP7 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62754-plasmid-map-sequence-id-230170

    Enlarge

  44. PHIP

    Plasmid
    #39049
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHIP (PHIP Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  45. TMPRSS2 g4 + Cas9

    Plasmid
    #153018
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153018-plasmid-map-sequence-id-298698

    Enlarge

  46. pDK714

    Plasmid
    #134230
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK365 tdTomato (short SKAP) (KNSTRN Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Available Since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134230-plasmid-map-sequence-id-272270

    Enlarge

  47. TPK1

    Plasmid
    #32865
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TPK1 (TPK1 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. pINDUCER20-FLAG-hMYF5 (#801)

    Plasmid
    #78334
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    MYF5 (MYF5 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Promoter
    TRE2
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  49. pRK VSV Cortactin

    Plasmid
    #50727
    Purpose
    mammalian expression of VSV-tagged Cortactin
    Depositor
    Kenneth Yamada
    Insert
    Cortactin (CTTN Human)
    Tags
    2xVSV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. pT7-7 asyn S87A/S129A

    Plasmid
    #36063
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87A/S129A (SNCA Human)
    Expression
    Bacterial
    Mutation
    S87A/S129A
    Promoter
    T7
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36063-plasmid-map-sequence-id-44519

    Enlarge

Showing: 4451 - 4500 of 9302 results