We narrowed to 157,076 results for: Des

1. KK402: pMVP/Zeo-DEST

   Plasmid

   #121859

   Purpose

   pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Desmoplakin II tension sensor (FL-based)

   Plasmid

   #118721

   Purpose

   The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-FL-mCherry

   Expression

   Mammalian

   Mutation

   inserted FL-based tension sensor module after aa1…

   Promoter

   CMV

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST15.1-hRPN13 (285-386)

   Plasmid

   #61936

   Purpose

   Expression of hRPN13 (285-386) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hRPN13 (285-386) (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Construct starts at residue 285, stop codon intro…

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDestTol2-actinb-kcnj13-EGFP

   Plasmid

   #164940

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   EGFP

   Mutation

   Stop codon deleted

   Promoter

   actinb (actb2) promoter

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMuLE Lenti Dest IFP1.4

   Plasmid

   #62177

   Purpose

   MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Lentiviral; Mule gateway destination vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti4/V5-DEST-P85(KS459del)

   Plasmid

   #40222

   Depositor

   Lynda Chin

   Insert

   P85(KS459delN) (PIK3R1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   amino acids 459-460 replaced with asparagine

   Promoter

   CMV

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA pDEST40 Cas9 GW STOP

   Plasmid

   #183196

   Purpose

   Destination vector

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST40-2XHA-Src-S32

   Plasmid

   #140308

   Purpose

   Expresses HA-tagged human Src-S32 in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH3 and SH2 domains

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMuLE Lenti Dest Luc2

   Plasmid

   #62179

   Purpose

   MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses firefly luciferase under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Luciferase; Mule gateway destination vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.2 V5-DEST mir-1-1 reporter

    Plasmid

    #46646

    Purpose

    expression of query pri-miRNA

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir-1-1 reporter
    

    Use

    RNAi; Expression of query pri-mirna sequences in …

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JT504: pMVP/SB/eGFP-DEST

    Plasmid

    #121861

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JT601: pMVP/SB/mCherry-DEST

    Plasmid

    #121862

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST-IRES-puro-polyA

    Plasmid

    #43967

    Depositor

    Peter Hohenstein

    Article

    Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

    Insert

    puroR
    

    Use

    Gateway dest vector

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti4/V5-DEST-P85(G376R)

    Plasmid

    #40220

    Depositor

    Lynda Chin

    Insert

    P85(G376R) (PIK3R1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Glycine 376 to Arginine

    Promoter

    CMV

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pET-DEST42-SARA-V5-His6

    Plasmid

    #67431

    Purpose

    Expression of hSara with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDest-686 (attR4-attR2)

    Plasmid

    #161889

    Purpose

    Multisite Gateway (attR4-attR2) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST40-2XHA-Src-S32-KD

    Plasmid

    #140310

    Purpose

    Expresses HA-tagged human Src-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Contains only the SH3, SH2, and KD regions

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDest-305 (attR4-attR2)

    Plasmid

    #161895

    Purpose

    Multisite Gateway (attR4-attR2) vector for mammalian protein expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/nV5-DEST-Apr1

    Plasmid

    #20135

    Depositor

    Valeri Vasioukhin

    Article

    Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

    Insert

    pcDNA3.1/nV5-DEST-Arp1 (Actr1a Mouse)
    

    Expression

    Mammalian

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pPB-DEST-R1R2-pNeo

    Plasmid

    #141057

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Neomycin resistance gene
    

    Expression

    Mammalian

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge