We narrowed to 7,572 results for: Lif

1. pGI3EM09

   Plasmid

   #135482

   Purpose

   Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   SpunHsp70pr hph-GFP ADH1t
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunHsp70pr

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ntn4-AP-His

   Plasmid

   #71980

   Purpose

   Expresses the entire Netrin 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntn4 (Ntn4 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CVS-N2c-tdTomato-FlpO

   Plasmid

   #172375

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   tdTomato + FlpO
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDL001_Hec1-ms_IgG_HC

   Plasmid

   #193618

   Purpose

   Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
   

   Use

   Tags

   His6 and signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDL002_Hec1-ms_IgG_LC

   Plasmid

   #193619

   Purpose

   Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 light chain (Hec1-ms_IgG_LC)
   

   Use

   Tags

   signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

   Plasmid

   #202568

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPI227

   Plasmid

   #113231

   Purpose

   safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct
   

   Use

   Safe haven knock-in plasmid for the act5 locus,li…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   act5

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE

   Plasmid

   #136916

   Purpose

   Expresses ChR2(H134R)-EYFP in Cre-negative cells

   Depositor

   Ofer Yizhar

   Article

   Sermet et al Elife. 2019 Dec 20;8. pii: 52665. doi: 1

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H134R in ChR2

   Promoter

   Human Synapsin1

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-Mito-CaNARi

   Plasmid

   #64851

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, N-terminal 30 amino acids from DAKAP1, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEM09

    Plasmid

    #135479

    Purpose

    Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr
    

    Use

    Tags

    GFP and hph

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Spun Hsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXWJ109AsAmp30E

    Plasmid

    #123144

    Purpose

    Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRXIP-GFP-STX17TMΔC

    Plasmid

    #221737

    Purpose

    Stable expression of GFP-STX17TMΔC in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    STX17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-228 and 227-302

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF(UG) hSyn PP-HaloTag-SYT7alpha

    Plasmid

    #179714

    Purpose

    Lentiviral expression of mouse Syt7 with N terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF(UG) hSyn PM-SYT7alpha deltaTMD

    Plasmid

    #179720

    Purpose

    Lentiviral expression of plasma membrane-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVtight-RFP-IRES-CD

    Plasmid

    #126678

    Purpose

    Doxycycline inducible expression of CD along with reporter RFP

    Depositor

    Joan Massague

    Article

    Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

    Insert

    RFP-IRES-CD (FCY1 Budding Yeast)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    tight TRE

    Available since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRv2 sgSp1

    Plasmid

    #174907

    Purpose

    Second generation sgRNA against Sp1

    Depositor

    Jane Clifford

    Article

    Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

    Insert

    Specificity Protein 1 (SP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Missense mutation to mutate PAM sequence

    Promoter

    EFS-NS

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF NS34a-msGFP-'S-mito

    Plasmid

    #158765

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CVS-N2c-tdTomato-ChIEF

    Plasmid

    #172370

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    tdTomato + ChIEF
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXWJ109HgAmp30E

    Plasmid

    #123149

    Purpose

    Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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