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pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

Plasmid

#164614

Purpose

Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.

Depositor

Jeffrey Chao

Article

Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

Insert

stdMCP-stdHalo-RH1

Use

Lentiviral

Tags

HA tag and NLS

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

Plasmid

#170229

Purpose

Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Q64L

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-NLS

Plasmid

#199663

Purpose

expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NLS and V5

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180266

Purpose

Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti4-Puro-H19

Plasmid

#200835

Purpose

Lentiviral vector expressing human long non-coding RNA H19

Depositor

Reinier Boon

Article

Hofmann et al Cardiovasc Res. 2019 Jan 1;115(1):230-24

Insert

Human long non-coding RNA H19

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_MYCN_P2A_Hygro_Barcode

Plasmid

#120463

Purpose

Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCN (MYCN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK Tgfbr3-HA neo

Plasmid

#58508

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Tgfbr3_HA (Tgfbr3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only
lenti-GBA-N370S-nick sgRNA

Plasmid

#214096

Purpose

Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G13A nicking sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROD1_P2A_Hygro_Barcode

Plasmid

#120466

Purpose

Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-nick sgRNA

Plasmid

#214097

Purpose

Lentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R nicking sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-T790M-nick sgRNA

Plasmid

#214098

Purpose

Lentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR T790M nicking sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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lenti F9-TetR-EGFP-IRES-PuroR

Plasmid

#117049

Purpose

lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Insert

F9-TetR-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

F9 promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 wt

Plasmid

#107104

Purpose

Lentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5c-Myc

Plasmid

#186616

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5c (KIF5C Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304 GDF11-V5 blast

Plasmid

#83098

Purpose

Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

GDF11 (GDF11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-myr-CSK-GFP

Plasmid

#83470

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

doxycycline-inducible promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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