We narrowed to 3,747 results for: mak

1. pAAV-2xflox-BRX-eGFP-NLS

   Plasmid

   #217535

   Purpose

   AAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivo

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   4X BRE reporter and miniXon casette (Smad1 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   BMP response element (BRE)

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MH6-RspWYL1

   Plasmid

   #108306

   Purpose

   Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-CMV-hAAT-W

   Plasmid

   #126704

   Purpose

   Constituitive, ubiquitious human alpha - 1 antitrypsin gene expression

   Depositor

   Darrell Kotton

   Article

   Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

   Insert

   hAAT (SERPINA1 Human)
   

   Use

   Lentiviral

   Promoter

   CMVp

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172503

   Purpose

   Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-Cdk9 T186A

   Plasmid

   #28101

   Depositor

   Andrew Rice

   Article

   Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

   Insert

   Cdk9 (CDK9 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Contains a T186A mutation in Cdk9

   Available Since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDF0352 HvsCas7-11 with C-term Nes mammalian expression

   Plasmid

   #172516

   Purpose

   Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   C-term Nes HvsCas7-11 mammalian expression
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRXIB-mRuby3-STX17TM

   Plasmid

   #221744

   Purpose

   Stable expression of mRuby3-STX17TM in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   STX17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-228

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.U6gLacZ.hSyn.flex.H2B.RFP

   Plasmid

   #170373

   Purpose

   Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AF1521-c002

   Plasmid

   #173109

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   KH-M1-L192

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AF1521-c003

    Plasmid

    #173111

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
    

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    KH-M1-L192

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRXIP-GFP-PI4KB

    Plasmid

    #221759

    Purpose

    Stable expression of GFP-PI4KB in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PI4KB (PI4KB Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3_5UTR_Myc_FLuc

    Plasmid

    #229502

    Purpose

    Myc 5' untranslated region firefly luciferase reporter.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    Myc 5'UTR
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMIII-Neo-BFP

    Plasmid

    #153426

    Purpose

    Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    mTagBFP2
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. RNF146-c005

    Plasmid

    #173091

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    RNF146 (RNF146 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    R99-L183, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GDAP2-c005

    Plasmid

    #173110

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GDAP2 (GDAP2 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    M1-N231

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Twitch-4 pcDNA3

    Plasmid

    #49533

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-4
    

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172505

    Purpose

    Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDF0336 pAAV EFS-HvsCas7-11 for dual vector

    Plasmid

    #172515

    Purpose

    Encodes HvsCas7-11 mammalian expression casette in AAV backbone

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV EFS-HvsCas7-11 for dual vector
    

    Use

    AAV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Twitch-1 pcDNA3

    Plasmid

    #49812

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-1
    

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac1-Ls-AChBP

    Plasmid

    #63126

    Purpose

    Expression of Lymnaea stagnalis AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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