165,652 results

1. pMax_ScaI_BFP

   Plasmid

   #177824

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP
   

   Expression

   Mammalian

   Mutation

   5' inserted ScaI RE cut site

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-SF3B1-K700E-reporter

   Plasmid

   #200318

   Purpose

   A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Insert

   BSD-2A-EGFPi
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-CBP core

   Plasmid

   #179543

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn1_nLightG

   Plasmid

   #187179

   Purpose

   Expresses green norepinephrine indicator nLightG in neuronal cells

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Has Service

   AAV9

   Insert

   nLightG
   

   Use

   AAV

   Tags

   Flag tag

   Promoter

   human Synapsin-1

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

   Plasmid

   #226515

   Purpose

   Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   Cox8a(4x)-SNAP-tag2-T2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBroad-T7RNAP-chlorR

   Plasmid

   #199992

   Purpose

   T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   T7RNAP Circuit
   

   Expression

   Bacterial

   Mutation

   copFnull

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

   Plasmid

   #205317

   Purpose

   All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISYmu1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-spCas9-N57

   Plasmid

   #169920

   Purpose

   Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-N57
   

   Use

   CRISPR

   Mutation

   N57 is the PAI domain of SB100X

   Promoter

   CAG

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-NLS-actin-R62D

   Plasmid

   #118381

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   NLS and R62D

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmEGFP_a_tubulin_C1

    Plasmid

    #21039

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Tags

    pmEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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