We narrowed to 9,182 results for: Pol;

1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

   Plasmid

   #129044

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA4 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

   Plasmid

   #129045

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA5 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

   Plasmid

   #129046

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA6 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-Flag-BRD4-WT

   Plasmid

   #90331

   Purpose

   expresses Wild type BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5-Flag-BRD4-BD

   Plasmid

   #90005

   Purpose

   expresses BD mutant BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed 140N to 140A, 433N to 433A

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZL Neo Myr Flag RPS6KB1

   Plasmid

   #20624

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   RPS6KB1 (RPS6KB1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pWZL Neo Myr Flag RPS6KB2

   Plasmid

   #20625

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   RPS6KB2 (RPS6KB2 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pWZL Neo Myr Flag IKBKE

   Plasmid

   #20504

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKBKE (IKBKE Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5-Flag-BRD4-7D

   Plasmid

   #90007

   Purpose

   expresses 7D mutant BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed 484S to 484D, 488S to 488D, 492S to 492D,…

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-Flag-BRD4-7A

    Plasmid

    #90006

    Purpose

    expresses 7A mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 484S to 484A, 488S to 488A, 492S to 492A,…

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid

    #52093

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid

    #52092

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGLU_82

    Plasmid

    #225783

    Purpose

    RS421 library variant 13550

    Depositor

    Nili Ostrov

    Insert

    13550_[abr]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGLU_100

    Plasmid

    #225813

    Purpose

    RS421 library variant 13550

    Depositor

    Nili Ostrov

    Insert

    13550_[Himar]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGLU_118

    Plasmid

    #225798

    Purpose

    RS421 library variant 13550

    Depositor

    Nili Ostrov

    Insert

    13550_[Tn5]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLfa-PUb-MCS

    Plasmid

    #52908

    Purpose

    Aedes aegypti polyubiquitin promoter followed by MCS

    Depositor

    Zach Adelman

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Ae aegptyi polyubiquitin

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOpen-T4gene43

    Plasmid

    #165524

    Purpose

    Polymerase that replicates viral genomic DNA.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T4 DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pOpen_taq

    Plasmid

    #153315

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    Drew Endy

    Insert

    Taq polymerase
    

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pOpen-T7gene1

    Plasmid

    #165505

    Purpose

    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T7 RNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    July 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pBS-3xpA

    Plasmid

    #176505

    Purpose

    3x SV40 polyA sequences

    Depositor

    Mario Capecchi

    Insert

    3x SV40 polyA
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBS-2xpA

    Plasmid

    #176504

    Purpose

    2x SV40 polyA sequences

    Depositor

    Mario Capecchi

    Insert

    2x SV40 polyA
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS-4xpA

    Plasmid

    #176506

    Purpose

    4x SV40 polyA sequences

    Depositor

    Mario Capecchi

    Insert

    4x SV40 polyA
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. L4437

    Plasmid

    #1652

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    RNAi

    Tags

    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, T7polN, T7…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCAG-VSVL

    Plasmid

    #64085

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

    Depositor

    Ian Wickersham

    Insert

    VSV polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJY4092

    Plasmid

    #113334

    Purpose

    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Insert

    codon-optimized Hermes transposase
    

    Expression

    Yeast

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. C95m

    Plasmid

    #121001

    Purpose

    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    T7 RNA polymerase (Andy please check!)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 371-A

    Plasmid

    #97026

    Purpose

    yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOpen-T5gene122

    Plasmid

    #165544

    Purpose

    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T5 DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. 371-U

    Plasmid

    #97036

    Purpose

    MYFQSNA-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    MYFQSNA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-B19L

    Plasmid

    #59922

    Purpose

    Expression vector for Rabies virus SAD B19 RNA dependent RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    Rabies virus RNA dependent RNA polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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