We narrowed to 24,628 results for: Nes

1. pETM6-C4-mCherry

   Plasmid

   #66530

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter C4

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - C4

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LAMP-OeNL_pcDNA3

   Plasmid

   #89525

   Purpose

   Orange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Use

   Tags

   LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKin1C

   Plasmid

   #31605

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   kinesin-1C (Kif5c Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PEP111E

   Plasmid

   #97397

   Purpose

   binary construct of plastid-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Plastid target

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMaCTag-Z20

   Plasmid

   #120063

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   10xSuntag
   

   Use

   Pcr template

   Tags

   10xSuntag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMaCTag-P09

   Plasmid

   #120020

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenus

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mVenus
   

   Use

   Pcr template

   Tags

   mVenus

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGLuc

   Plasmid

   #135288

   Purpose

   GBPart - GLuc Luciferase CDS

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   Gaussia Luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Nano-lantern(Ca2+)_150/pcDNA3

   Plasmid

   #51984

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-2G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. Nano-lantern(Ca2+)_11/pcDNA3

   Plasmid

   #51987

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-4GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pUF-AsCpf1-pre-gRNA

    Plasmid

    #137849

    Purpose

    AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    AsCpf1 and pre-gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Nano-lantern-mitochondria/pcDNA3

    Plasmid

    #51973

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pUF-LbCpf1-pre-gRNA

    Plasmid

    #137848

    Purpose

    LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    LbCpf1-NLS-FLAG and gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TET-pLKO.1 PURO shGFP

    Plasmid

    #83085

    Purpose

    Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pMaCTag-P25

    Plasmid

    #120036

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    YFP-LID
    

    Use

    Pcr template

    Tags

    YFP-LID

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPnos (GB0072)

    Plasmid

    #68166

    Purpose

    Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Nos promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMaCTag-Z11

    Plasmid

    #120054

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet-i
    

    Use

    Pcr template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMaCTag-P02

    Plasmid

    #120013

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TagBFP
    

    Use

    Pcr template

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PEP113E

    Plasmid

    #97399

    Purpose

    binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Peroxisome target

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330A_D10A-1x4

    Plasmid

    #58774

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TET-pLKO.1 PURO shLacZ

    Plasmid

    #98632

    Purpose

    Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLacZ
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GL2 shRNA

    Plasmid

    #86083

    Purpose

    A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    control shRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDGB3_alpha1

    Plasmid

    #68228

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDsRed (GB0100)

    Plasmid

    #68202

    Purpose

    Provides the CDS of DsRed as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    DsRed coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSLIK 3xFLAG-MTUS1 zeo

    Plasmid

    #192323

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-MTUS1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    MTUS1 (MTUS1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMaCTag-Z09

    Plasmid

    #120052

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mVenus
    

    Use

    Pcr template

    Tags

    mVenus

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330A_D10A-1x6

    Plasmid

    #58776

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMaCTag-Z17

    Plasmid

    #120060

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mEos4b
    

    Use

    Pcr template

    Tags

    mEos4b

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMaCTag-P14

    Plasmid

    #120025

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeptune2.5
    

    Use

    Pcr template

    Tags

    mNeptune2.5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-Venus-Emi1 (914)

    Plasmid

    #39854

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

    Insert

    Emi1 (FBXO5 Human)
    

    Use

    In vitro transcription

    Tags

    Venus

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p3XP3-WhiteA2

    Plasmid

    #165875

    Purpose

    Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Eye Marker Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMaCTag-P15

    Plasmid

    #120026

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMaCTag-Z33

    Plasmid

    #120050

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    FLAG
    

    Use

    Pcr template

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111926

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTRF.1-mCMV-udsVenus

    Plasmid

    #58518

    Purpose

    Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    None
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Nano-lantern(Ca2+)_600/pcDNA3

    Plasmid

    #51982

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-2G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pMaCTag-P24

    Plasmid

    #120035

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TAP
    

    Use

    Pcr template

    Tags

    TAP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMaCTag-P10

    Plasmid

    #120021

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKOk
    

    Use

    Pcr template

    Tags

    mKOk

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111928

    Purpose

    Orange color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMaCTag-P18

    Plasmid

    #120029

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge