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pLV-CDH5-mRuby3-IRES-puro

Plasmid

#85144

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)

Use

Lentiviral

Tags

mRuby3

Promoter

CAG

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Publication

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CAG-Flex-RG

Plasmid

#48333

Purpose

Cre-dependant (flex) rabies glycoprotein

Depositor

Liqun Luo

Publication

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

Rabies Glycoprotein

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

Plasmid

#107387

Purpose

Lentiviral expression of IKZF1-V1-FLAG

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Publication

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Expression

Bacterial

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-eGFP-W

Plasmid

#126686

Purpose

Constitutive, ubiquitous GFP expression.

Depositor

Darrell Kotton

Publication

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

eGFP

Use

Lentiviral

Promoter

EF1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMx-puro-MGT

Plasmid

#111809

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Publication

Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

Insert

Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

viral LTR

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

Plasmid

#132400

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Publication

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RFP Dynamin2 K44A

Plasmid

#128153

Purpose

Expression of Dynamin 2 K44A

Depositor

Jennifer Lippincott-Schwartz

Publication

Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dynamin 2 (Dnm2 Rat)

Expression

Mammalian

Mutation

K44A

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEXP(V5_H2B_APEX2)

Plasmid

#107597

Purpose

Expresses V5_H2B_APEX2 in mammallian cells

Depositor

Markus Hafner

Publication

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-APEX2

Tags

H2B and V5

Expression

Mammalian

Promoter

CMV Dox inducible

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pECGMC3

Plasmid

#75441

Purpose

Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

Depositor

James Collins

Publication

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Expression

Bacterial

Promoter

nisA promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plenti-UbcP-HA-CRBN-pGK-HYG

Plasmid

#107378

Purpose

Lentiviral expression of HA-CRBN

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UbcP

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Publication

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK3

Plasmid

#122674

Purpose

Expresses mouse PCSK3 in mammalian cells

Depositor

Liming Pei

Publication

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-VARNAM

Plasmid

#115552

Purpose

Red fluorescent voltage indicator for mammalian expression

Depositor

Vincent Pieribone

Publication

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VARNAM

Tags

Golgi and ER export signal

Expression

Mammalian

Promoter

CMV IE94

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Publication

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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