We narrowed to 2,128 results for: Pam

1. pLPT234

   Plasmid

   #127855

   Purpose

   Repressilator 2.0, without degradation tags, integrated triple reporter

   Depositor

   Johan Paulsson

   Article

   Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

   Inserts

   bla
   mSCFP3
   mKate2
   mVenus
   TetR
   LacI
   cI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   First 11aa derived from mCherry as fusion protein

   Promoter

   pAmpR, pL LacO1, pL tetO1, and pR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IZ-SEC61B-mCherry-FRB

   Plasmid

   #233349

   Purpose

   Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   SEC61B (SEC61B Human)
   mCherry
   FRB
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   T2098L mutant of FKBP-rapamycin binding domain of…

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP-CD28TMD-NTEVp(H75E)

   Plasmid

   #137829

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75E)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-Lck-FRB-mTurquoise2

   Plasmid

   #176130

   Purpose

   Plasma membrane localized FRB, rapamycin heterodimerization

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   FRB
   

   Use

   Lentiviral

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRB-mCherry-EEA1

   Plasmid

   #222419

   Purpose

   To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   EEA1 (EEA1 Human)
   

   Tags

   FRB-mCherry

   Expression

   Mammalian

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST_DSUP_RAMVA (pBS0353)

   Plasmid

   #185189

   Purpose

   For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DSUP_RAMVA
   

   Expression

   Mammalian

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP3-bio

   Plasmid

   #47731

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP3
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. A2M-bio-His

   Plasmid

   #53423

   Purpose

   Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   A2M (A2M Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Keasling-2187

   Plasmid

   #100167

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   MevT and MBIS operon
   

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST_CAHS3_HYPDU (pBS0333)

    Plasmid

    #185179

    Purpose

    For the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS3_HYPDU
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST_APOE_HUMAN_E114I (pBS0839)

    Plasmid

    #185274

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_E114I
    

    Expression

    Mammalian

    Mutation

    E114I

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST_AfrLEA3m (pBS0911)

    Plasmid

    #185285

    Purpose

    For the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    AfrLEA3m
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST_PvLEA4_repeats_k3_26 (pBS0762)

    Plasmid

    #185244

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Expression

    Mammalian

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST_SERF2_HUMAN (pBS0751)

    Plasmid

    #185239

    Purpose

    For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    SERF2_HUMAN
    

    Expression

    Mammalian

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pST_MAHS_RAMVA (pBS0310)

    Plasmid

    #185169

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST_DHN_AtCor47 (pBS0784)

    Plasmid

    #185254

    Purpose

    For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DHN_AtCor47
    

    Expression

    Mammalian

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP-YPet-RanGAP*

    Plasmid

    #175245

    Purpose

    Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. alpha1Ddelta-G12-FRB

    Plasmid

    #87454

    Purpose

    rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    a1D short variants (Cacna1d Rat)
    

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    delete KYPAKNTTIALQMLERML

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-CAG-GFP

    Plasmid

    #243759

    Purpose

    AAV plasmid expressing GFP under the CAG promoter.

    Depositor

    Andrew Arrant

    Article

    Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Stargazin-EBFP2-FRB

    Plasmid

    #222417

    Purpose

    To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    Cacng2 (Cacng2 Mouse)
    

    Tags

    EBFP2-HA-FRB

    Expression

    Mammalian

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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