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pFS461

Plasmid

#89064

Purpose

leu1 integration plasmid with adh1pr:Z3EV

Depositor

Nick Rhind

Publication

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV artificial transcription factor

Use

Leu1 integrating vector

Promoter

adh1

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLPC-LaminA

Plasmid

#69059

Purpose

encodes the LaminA-CS protein

Depositor

Gerardo Ferbeyre

Publication

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

LaminA-CS (LMNA Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAAV-Ef1a-Coff/Fon-oScarlet

Plasmid

#137138

Purpose

Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Coff/Fon-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-RapR-FAK-YM

Plasmid

#25928

Depositor

Klaus Hahn

Publication

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Human, Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EF1a-dCasRx-RBM25

Plasmid

#221001

Purpose

Transient expression of dCasRx-RBM25. EF1a promoter.

Depositor

Benjamin Blencowe, Mikko Taipale

Publication

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

dCasRx-RBM25

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Promoter

EF1a

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-HygR

Plasmid

#118153

Purpose

SpCas9 expression vector

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

hSpCas9-T2A-HygR

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CAG and U6

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFS478

Plasmid

#89066

Purpose

KanMX Z3EV promoter cassette

Depositor

Nick Rhind

Publication

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV promoter cassette

Use

Yeast integration vector

Mutation

yeast Gal1 promoter modified with multiple Zif268…

Promoter

Z3EV promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p44K

Plasmid

#45800

Depositor

Luc Ponchon

Publication

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJOE7706.1

Plasmid

#135075

Purpose

A shuttle vector for heterologous HA production with Corynebacterium glutamicum

Depositor

Josef Altenbuchner

Publication

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Type

Empty backbone

Expression

Bacterial

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(-) FLIPQTV3.0 2m

Plasmid

#63731

Purpose

FLIPQTV3.0 glutamine sensor with R75MW220A mutations

Depositor

Sakiko Okumoto

Publication

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 2mM affinity

Expression

Mammalian

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFastBac-M2

Plasmid

#135583

Purpose

High eukaryotic protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Publication

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAGGS-PKCdelta-EGFP

Plasmid

#178856

Purpose

Mammalian expression of PKCδ fused to EGFP

Depositor

Shinya Tsukiji

Publication

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ (PRKCD Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

Plasmid

#137150

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Coff/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 S15D

Plasmid

#69005

Purpose

mammalian expression of human p53 S15D mutant

Depositor

David Meek

Publication

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

S15D

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pIRES2-mCherry-p53 deltaN

Plasmid

#49243

Purpose

expresses human p53 deltaN and mCherry in mammalian cells

Depositor

Dylan Taatjes

Publication

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN (TP53 Human)

Tags

IRES-mCherry

Expression

Mammalian

Mutation

deltaN ( lacks 39 residues at the N-terminus)

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pXT130_TwinStrep-SUMO-ShTnsC

Plasmid

#135526

Purpose

Protein expression construct for ShTnsC

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsC

Tags

TwinStrep-SUMO

Expression

Bacterial

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(-) FLIPQTV3.0 100micro

Plasmid

#63730

Purpose

FLIPQTV3.0 glutamine sensor with D157N mutation

Depositor

Sakiko Okumoto

Publication

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 100microM affinity

Expression

Mammalian

Mutation

D157N in glutamine binding domain

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-hCDC6p-Venus-puro

Plasmid

#212666

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Publication

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Promoter

hCDC6p

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CaMKKbeta

Plasmid

#32453

Depositor

Anthony Means

Publication

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Tags

FLAG and GFP

Expression

Mammalian

Promoter

CMV

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Publication

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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