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JDW 1042 (pLenti6.2_CMV_V5_TCF7L1_P2A_H2A_mCherry)

Plasmid

#233540

Purpose

A CMV driven human TCF7L1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L1 (TCF7L1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1039 (pLenti6.2_CMV_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#233539

Purpose

A CMV driven human TCF7 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1045 (pLenti6.2_CMV_V5_TCF7L2_P2A_H2A_mCherry)

Plasmid

#233541

Purpose

A CMV driven human ZIC1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

ZIC1 (ZIC1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#233538

Purpose

A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark3

Plasmid

#233145

Purpose

Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark3

Use

Prokaryotic expression

Tags

3x Ty tag

Expression

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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PHB2 S39A-mCherry

Plasmid

#232786

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2 non-phosphorylatable on Ser39

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed Ser 39 to Ala

Promoter

CMV

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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PHB2 S39D-mCherry

Plasmid

#232787

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 39 to Aspartate

Promoter

CMV

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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Ubi:ccdb-mTb + mcherry

Plasmid

#233305

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of miniTurbo-ID paired with ccdb selection toxin and mCherry marker

Depositor

Thomas Baum

Insert

Ubi:ccdb-mTb + mcherry

Use

Gateway destination

Tags

miniTurbo-ID

Expression

Mutation

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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Ubi:mTb-ccdb + mcherry

Plasmid

#233304

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of miniTurbo-ID paired with ccdb selection toxin and mCherry marker

Depositor

Thomas Baum

Insert

Ubi:mTb-ccdb + mcherry

Use

Gateway destination

Tags

miniTurbo-ID

Expression

Mutation

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)

Plasmid

#233542

Purpose

A CMV promoter driving expression of a murine dominant negative myc tagged TCF4.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF4 (TCF4 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mutation

lacks aa 1-56

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#233545

Purpose

A CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

Plasmid

#232128

Purpose

A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA2 (GATA2 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#232124

Purpose

A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1035 (pME_V5_TCF7L1_P2A_H2A_mCherry)

Plasmid

#232125

Purpose

A middle entry gateway clone containing V5 tagged human TCFL7 / TCF3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L1 / TCF3 (TCF7L1 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#232129

Purpose

A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

Plasmid

#232131

Purpose

A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF2 (FOXF2 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

Plasmid

#232130

Purpose

A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 1036 (pME_V5_TCF7L2_P2A_H2A_mCherry)

Plasmid

#232126

Purpose

A middle entry gateway clone containing V5 tagged human TCF7L2 / TCF4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L2 / TCF4 (TCF7L2 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-pHluorin-His

Plasmid

#231546

Purpose

Expresses an mCherry-pHluorin fusion protein in bacteria, with His tag.

Depositor

David O'Connor

Insert

mCherry-pHluorin

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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