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pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

Plasmid

#175534

Purpose

Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag7-myc_PDGFRtmb

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-CKS1

Plasmid

#177013

Purpose

Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CKS1 (CKS1B Synthetic, Human)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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GLuc-FLAG-CB1

Plasmid

#158066

Purpose

Membrane expression detection of Cannabinoid receptor 1

Depositor

Adolfo Rivero-Muller

Article

Rodriguez-Rodriguez et al Sci Rep. 2020 Oct 23;10(1):18191. doi: 1

Insert

CB1 (CNR1 Human)

Use

AAV

Tags

Gaussia Luciferase

Expression

Mammalian

Mutation

contains 4Kb of 3' UTR

Promoter

pCAG

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-BLOS1

Plasmid

#164625

Purpose

Expresses BLOS1 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

BLOS1 (BLOC1S1 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-SNAP

Plasmid

#175295

Purpose

Track mature FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with SNAP-tag

Tags

SNAP-tag

Expression

Mammalian

Available Since

Oct. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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hSYN-mito-dsRED2

Plasmid

#173069

Purpose

mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Mito

Use

Lentiviral

Tags

dsRED

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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DDX41-GFP

Plasmid

#175494

Purpose

Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.

Depositor

Evan Braunstein

Article

Li et al Haematologica. 2016 Jun;101(6):e228-31.

Insert

DDX41 (DDX41 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC3A2_STOP

Plasmid

#161379

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PB_iNEUROD1_P2A_GFP_Puro

Plasmid

#168803

Purpose

PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

NEUROD1 (NEUROD1 Synthetic, Human)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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HA-HA-AR-DBD-LBD

Plasmid

#171237

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)

Tags

HA

Expression

Bacterial and Mammalian

Mutation

AR truncate

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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