Addgene

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TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Use

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pET28-sfGFP[D134TAG+N150TAA]

Plasmid

#164582

Purpose

superfolder GFP with D134TAG and N150TAA mutations

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-134TAG+150TAA

Use

Tags

6xHis

Expression

Bacterial

Mutation

D134TAG + N150TAA Mutations

Promoter

T7

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-1

Plasmid

#126053

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1202

Plasmid

#44484

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, MCS

Use

Multiple cloning site vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2b1

Plasmid

#13756

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b1 (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Use

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBstSK+IRES-V5-IPpoI (mutant)

Plasmid

#206322

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag-mutated IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein (mutant version)

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_D27NDHFR_WTTS

Plasmid

#91244

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

D27N

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31YG121VDHFR_WTTS

Plasmid

#91229

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121V

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_G121VDHFR_WTTS

Plasmid

#91233

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

G121V

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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deltaPYD-NLRP11.MYC

Plasmid

#131197

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

deltaPYD-NLRP11 (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

deletion of the pyrin domain (amino acids 1-124 m…

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Use

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/3xmyc_NLRP11-LRR

Plasmid

#131200

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

LRR of NLRP11 (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

contains only the leucine-rich repeats (LRRs), am…

Promoter

pCMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1001

Plasmid

#44476

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pET24a-AlkB deltaN11

Plasmid

#73622

Purpose

Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

Depositor

Catherine Drennan

Article

Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

AlkB (alkB E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

deletion of first 11 AA

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
LifeAct-TagBFP-DiB-RM

Plasmid

#168782

Purpose

Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

LifeAct-TagBFP-DiB-RM

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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ATF4 18: RLuc.5’ATF4.GFP

Plasmid

#21864

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-NRASshort

Plasmid

#13799

Depositor

Frank Slack

Article

Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

Insert

NRAS (NRAS Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pCFJ1002

Plasmid

#44477

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

Plasmid

#52895

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[1]

Use

Tags

L-IFP-F[1]

Expression

Yeast

Mutation

Promoter

GAL1

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
MTK2_006

Plasmid

#123701

Purpose

Encodes pSV40 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSV40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NEW-fRGECO2

Plasmid

#125248

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Use

Tags

NES signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Use

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 S449A/T450A/S454A

Plasmid

#19845

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pGL4 mIL-17 1.1kb promoter

Plasmid

#20125

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pONSY-mCherry

Plasmid

#111874

Purpose

This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Expression

Mutation

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

myc eGFP 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_004

Plasmid

#123785

Purpose

Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mAzamiGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ907

Plasmid

#44473

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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