We narrowed to 19,465 results for: ATO

1. pET28_6xHis-PARP1-CATdeltaHD

   Plasmid

   #174811

   Purpose

   Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CATdeltaHD (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residues 678-787 replaced by eight-residue linker…

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEA01

   Plasmid

   #170766

   Purpose

   Utilisation of pSAM2 excision tool in Amycolatopsis spp

   Depositor

   Jean-Luc Pernodet

   Article

   Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

   Inserts

   xis, int
   oriT
   pac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trcp

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-OsTIR1(F74A)

   Plasmid

   #140731

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74A)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2R3a-nosT

   Plasmid

   #71272

   Purpose

   Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   nopaline synthase terminator
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_6xHis-PARP1-CAT-L713F

   Plasmid

   #173944

   Purpose

   Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9-EF-5Nanog_2

   Plasmid

   #174871

   Purpose

   CRISPR vector for generating Nanog STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Nanog (Nanog Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBC-LG3-tdT

   Plasmid

   #62810

   Purpose

   pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

   Plasmid

   #140535

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. eSpCas9-EF-5Pou5f1

   Plasmid

   #174872

   Purpose

   CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

    Plasmid

    #153071

    Purpose

    Lentiviral vector expressing human ZC3H12D mutated (D95N)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Mutated ZC3H12D (D95N) (ZC3H12D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed D95 to N, known to abrogate the RNase enz…

    Promoter

    EF1α

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLC-HA active Nedd8-P2A-Hygro

    Plasmid

    #124307

    Purpose

    Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    Nedd8 (NEDD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Five C-terminal amino acids missing compared to w…

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2R3a-3AT

    Plasmid

    #71273

    Purpose

    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK322 (TOP2A-mAC (Hygro))

    Plasmid

    #140653

    Purpose

    TOP2A tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLC-CUL4A WT-P2A-Puro

    Plasmid

    #124304

    Purpose

    Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVTHM-shRnd3

    Plasmid

    #86437

    Purpose

    2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

    Depositor

    Violaine Moreau

    Article

    Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

    Insert

    shRNA targeting RND3 (RND3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-PARP1-L713F

    Plasmid

    #174794

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_6xHis-PARP1-C125G

    Plasmid

    #174792

    Purpose

    Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-C125G (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C125G, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-Basic-hCYP26A1-FL- luciferase

    Plasmid

    #135566

    Purpose

    Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

    Insert

    Full-length promoter of human retinoic acid receptor-beta gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    hHuman CYP26A1 gene promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. YFP-mDia2

    Plasmid

    #25420

    Depositor

    Arthur Alberts

    Article

    Wallar et al J Biol Chem. 2006 Feb 17. 281(7):4300-7.

    Insert

    diap3 (Diaph3 Mouse)
    

    Use

    Tags

    YFP A206K

    Expression

    Mammalian

    Mutation

    Encodes mDia2 amino acids T47 to L1171

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only