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We narrowed to 21,502 results for: SPR

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Showing: 3721 - 3760 of 21502 results

  4. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68417-plasmid-map-sequence-id-120195

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  10. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  11. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and EF1-a
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  14. pLM-AMA18.0 dCas9-VPR

    Plasmid
    #138945
    Purpose
    Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138945-plasmid-map-sequence-id-366628

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  16. pHAGE-BFP

    Plasmid
    #106282
    Purpose
    A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106282-plasmid-map-sequence-id-205458

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  18. pV1200

    Plasmid
    #111429
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111429-plasmid-map-sequence-id-212464

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  24. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

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  27. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158408-plasmid-map-sequence-id-330919

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  31. pYPQ-ABE-Cas9n-Act3.0

    Plasmid
    #178956
    Purpose
    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178956-plasmid-map-sequence-id-354328

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  32. pAH237

    Plasmid
    #121146
    Purpose
    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast
    Depositor
    Katsunori Tanaka
    Article
    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
    Inserts
    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    Use
    CRISPR
    Tags
    Flag
    Expression
    Yeast
    Promoter
    S.pombe rrk1 and nmt41
    Available Since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121146-plasmid-map-sequence-id-234796

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  40. pJH2970

    Plasmid
    #100954
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available Since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100954-plasmid-map-sequence-id-193247

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Showing: 3721 - 3760 of 21502 results