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We narrowed to 8,775 results for: construct

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Showing: 3721 - 3760 of 8775 results

  3. pGBW-m4046908

    Plasmid
    #145603
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145603-plasmid-map-sequence-id-298366

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  4. pGBW-m4046878

    Plasmid
    #145743
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145743-plasmid-map-sequence-id-298347

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  5. pGBW-m4046904

    Plasmid
    #145591
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145591-plasmid-map-sequence-id-298327

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  16. p5E-Ui4-eSIBR

    Plasmid
    #82586
    Purpose
    5'entry vector containing human ubiquitin C promoter followed by intronically-expressed eSIBR cassette for ubiquitous expression, RNAi
    Depositor
    Kryn Stankunas
    Article
    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi
    Insert
    human ubiquitin C promoter followed by intronically expressed eSIBR cassette
    Promoter
    None
    Available Since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    82586-plasmid-map-sequence-id-161213

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  18. pADH118-17

    Plasmid
    #91727
    Purpose
    NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91727-plasmid-map-sequence-id-180525

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  19. pADH143-2

    Plasmid
    #90990
    Purpose
    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90990-plasmid-map-sequence-id-180523

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  22. pHJ42

    Plasmid
    #48359
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48359-plasmid-map-sequence-id-70049

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  25. pBBM

    Plasmid
    #243675
    Purpose
    Expresses a maize optimized CDS of BABY BOOM in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator
    Depositor
    Patrick Krysan
    Article
    De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.
    Insert
    Maize optimized CDS of BABY BOOM
    Expression
    Plant
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    243675-plasmid-map-sequence-id-491734

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  27. pHYG

    Plasmid
    #243678
    Purpose
    Expresses the HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator
    Depositor
    Patrick Krysan
    Article
    De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.
    Insert
    HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS
    Expression
    Plant
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    243678-plasmid-map-sequence-id-491547

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  28. mGreenLantern-C OR (T AND V)-CAAX

    Plasmid
    #243791
    Purpose
    Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from membranes in response to HRV-3C OR (TEV AND TVMV) treatment. Construct contains a CAAX tag for intracellular membrane tethering.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    mGreenLantern
    Use
    Lentiviral
    Tags
    CAAX Tag
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243791-plasmid-map-sequence-id-486449

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  29. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

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  31. eSrtA-MT4

    Plasmid
    #200306
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT4 (MT4 Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200306-plasmid-map-sequence-id-394863

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  32. eSrtA-MT3

    Plasmid
    #200305
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT3 (MT3 Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200305-plasmid-map-sequence-id-394866

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  33. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193318
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Tags
    4MYC
    Expression
    Bacterial
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193318-plasmid-map-sequence-id-383635

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  34. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193317
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Tags
    4FLAG
    Expression
    Bacterial
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193317-plasmid-map-sequence-id-383630

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Showing: 3721 - 3760 of 8775 results