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pLKO.1-puro-cfPar3_4

Plasmid

#26706

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Par3 RNAi

Available since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
M20 siamois-luciferase p01234

Plasmid

#17157

Depositor

Randall Moon

Publication

Brannon et al Genes Dev. 1997 Sep 15. 11(18):2359-70.

Insert

siamois promoter (sia1.L Frog)

Use

Luciferase

Tags

luciferase

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pMT-HuC:Dendra2

Plasmid

#80904

Purpose

Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

Depositor

Periklis Pantazis

Publication

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2 (elavl3 Synthetic)

Use

Zebrafish expression

Promoter

HuC

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Actinin-sstFRET-GR

Plasmid

#83416

Purpose

FRET-based actinin tension sensor

Depositor

Tetsuya Kitaguchi

Publication

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Insert

Actinin-sstFRET-GR

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGinB-8X(GGS)-dCas9-FLAG-NLS

Plasmid

#81205

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

Depositor

David Liu

Publication

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pIntegrase_9

Plasmid

#60580

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_9

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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RP1B-PP1alpha

Plasmid

#51768

Purpose

Expresses PP1 alpha residues 7-330

Depositor

Wolfgang Peti

Publication

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

pp1 alpha

Tags

His, TEV protease site, and Thio-6

Expression

Bacterial

Mutation

Contains PP1 alpha residues 7-330

Promoter

T7-lac

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBP-p65AD::CaM

Plasmid

#64719

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Publication

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

p65AD::CaM

Expression

Insect

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

Plasmid

#71828

Purpose

Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

U6

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Publication

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX-GP-2-KDM4A double Tudor

Plasmid

#59699

Purpose

Contains histone modification interacting domain (KDM4A double Tudor)

Depositor

Albert Jeltsch

Publication

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

KDM4A double Tudor (KDM4A Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Publication

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Tags

GST

Expression

Bacterial

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCFJ1272

Plasmid

#44486

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, MCS

Use

Multiple cloning site vector

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M3499 ura3::ADE2 Disruptor Converter

Plasmid

#51674

Purpose

Yeast marker swap plasmid for converting URA3 to ADE2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Promoter

ADE2

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV-MAVS(KaganE17)

Plasmid

#52008

Purpose

Expresses the human MAVS CDS

Depositor

Jonathan Kagan

Publication

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Retroviral

Expression

Mammalian

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRPR1_c3gRNA_RPR1t

Plasmid

#64381

Purpose

encodes c3 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c3 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRS415-recR

Plasmid

#49457

Purpose

single copy recombinase yeast expression plasmid

Depositor

Marc Gartenberg

Publication

Ansari et al Methods. 1999 Feb;17(2):104-11.

Insert

recR

Expression

Yeast

Promoter

Gal1

Available since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pAS158

Plasmid

#194481

Purpose

synMR Part (Part 3)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synMR (Part 3)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS156

Plasmid

#194479

Purpose

synER Part (Part 3)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synER (Part 3)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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