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We narrowed to 9,096 results for: sgRNA

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Showing: 1861 - 1880 of 9096 results

  3. U6-sgRNA-BbsI-CMV-EGFP cloning vector

    Plasmid
    #239464
    Purpose
    sgRNA cloning vector with EGFP expression cassette. An sgRNA spacer sequence can be cloned into BbsI sites. A CMV-EGFP expression cassette is included as a reporter of transfection efficiency.
    Depositor
    Patrick Harrison
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 and CMV
    Available since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    239464-plasmid-map-sequence-id-476201

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  5. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid
    #122090
    Purpose
    U6 driven SaCas9 sgRNA expression vector for cloning own guides
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122090-plasmid-map-sequence-id-242296

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  8. px330-GAPDH

    Plasmid
    #136940
    Purpose
    NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GAPDH (GAPDH Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136940-plasmid-map-sequence-id-272092

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  10. pRCA377 - pBA904 Puro-T2A-GFP CD55 CRISPRa guide 1 (pRCA360 backbone)

    Plasmid
    #238168
    Purpose
    Lentiviral CRISPR guide vector expressing CD55 targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Publication
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    CD55 sgRNA CRISPRa (CD55 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238168-plasmid-map-sequence-id-474466

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  11. pX330-PITCh-TOMM20

    Plasmid
    #207789
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Dec. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207789-plasmid-map-sequence-id-455792

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  15. pX330 _CRISPIE donor B10

    Plasmid
    #172851
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172851-plasmid-map-sequence-id-341020

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  19. hPLD4-sgRNA-Cas9_mcherry

    Plasmid
    #199343
    Purpose
    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    sgRNA: accagtagtatgaagccacg
    Promoter
    human U6
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199343-plasmid-map-sequence-id-390620

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  20. pX330-NQL007-NANOG-sgRNA

    Plasmid
    #175555
    Purpose
    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.
    Depositor
    Elzo de Wit
    Publication
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175555-plasmid-map-sequence-id-345077

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Showing: 1861 - 1880 of 9096 results