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Showing: 1881 - 1900 of 13295 results

  8. pdnCas3-CDA1

    Plasmid
    #229535
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229535-plasmid-map-sequence-id-459013

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  9. pdnCas3-APOBEC1

    Plasmid
    #229537
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229537-plasmid-map-sequence-id-459005

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  13. LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3

    Plasmid
    #192397
    Purpose
    To test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).
    Depositor
    Ryan Lister
    Article
    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038
    Insert
    35S::FRT-OCS-FRT::B3RT-OCS-B3RT::Turbo GFP
    Use
    Synthetic Biology
    Tags
    N7
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192397-plasmid-map-sequence-id-378296

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  14. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

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  15. pCMV_FLAG-NFL(K363TAG)

    Plasmid
    #182661
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K363 & an N-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for genetic code expansion & click labeling of NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K363TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182661-plasmid-map-sequence-id-363012

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  17. pLV-MNTS-1-p75

    Plasmid
    #182945
    Purpose
    To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTR
    Depositor
    Pantelis Tsoulfas
    Article
    Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.
    Insert
    MNTS-1-p75 (NTF3, NT3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter and enhancer
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182945-plasmid-map-sequence-id-360103

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Showing: 1881 - 1900 of 13295 results