We narrowed to 7,506 results for: PAC;

1. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

   Plasmid

   #174081

   Purpose

   Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
   

   Use

   Tags

   NES-Flag

   Expression

   Mammalian

   Mutation

   L270G, Y271L, N311G, C313A

   Promoter

   CMV

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-DIO-EF1α-mKate2-biPAC

   Plasmid

   #169127

   Purpose

   AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

   Depositor

   Georg Nagel

   Article

   Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

   Insert

   mKate2-biPAC
   

   Use

   AAV and Cre/Lox

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   codon optimized to mouse

   Promoter

   EF1a

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-fDIO-pAce-Kv2.1PR

   Plasmid

   #195535

   Purpose

   Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAce-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Ace-mNeon 78K, 81D, 92N, 178F; SY linker

   Promoter

   CAG

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1

   Plasmid

   #58437

   Purpose

   Expresses LexA-ER-B112 in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   LexA-ER-B112
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Act1 promoter (-520;1)

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKRG3-mU6-PUb-hSpCas9-pAc

   Plasmid

   #162163

   Purpose

   Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   T2A-pAc

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

   Plasmid

   #58431

   Purpose

   Expresses LexA-ER-B42 in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   LexA-ER-B42
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Act1 promoter (-520;1)

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-mGFAP(ABC1D)-2pabPAC(F198Y)

   Plasmid

   #234548

   Purpose

   to express a version with reduced constitutive activity (thanks to point mutation F198Y) of the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

   Depositor

   Hajime Hirase

   Article

   Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

   Insert

   2pabPAC(F198Y)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   F198Y

   Promoter

   mGFAP(ABC1D)

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-mEGFP(A206K)-paCaMKII(K42M)

    Plasmid

    #165440

    Purpose

    Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)
    

    Use

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    K42M: Kinase dead mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFGC5941-PacI-ath-STTM156/157

    Plasmid

    #84083

    Purpose

    target miRNA156/157 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM156/157
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-MPAct-mRuby3-IRES-Puro

    Plasmid

    #155231

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mRuby sensor
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid

    #169552

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-NanoLuc Luciferase - mTagBFP fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. [pSK24] pACYCDuet His-c7orf59|HBXIP

    Plasmid

    #136143

    Purpose

    bacterial expression of c7orf59 and HBXIP

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183606

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Use

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Pgk1

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-DIO-pAceR-Kv2.1PR

    Plasmid

    #195534

    Purpose

    Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR-Kv2.1 proximal restriction sequence
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPyCAG-mKate2-3xNLS-IRES-Pac

    Plasmid

    #183618

    Purpose

    Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

    Insert

    mKate2-3xNLS
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC150-PBLHL-4xHS-EF1a-DEST

    Plasmid

    #48234

    Purpose

    PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-mEGFP(A206K)-paCaMKII-ShadowG

    Plasmid

    #167475

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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