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pGenLenti V5-NIX (E72A/L75A/D77A/E81A; deltaWIPI2)

Plasmid

#223789

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) (BNIP3L Human)

Use

Lentiviral

Tags

V5

Mutation

E72A/L75A/D77A/E81A; disrupts WIPI2 binding

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa)

Plasmid

#223760

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

Plasmid

#223762

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

Plasmid

#223766

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

EGFP-TEV-GST

Expression

Insect

Mutation

W18A/L21A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI4

Plasmid

#223769

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI4 (WDR45 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

Plasmid

#223738

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

W36A/L39A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

Plasmid

#223745

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BCL2L13 soluble part (BCL2L13 Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP

Plasmid

#223214

Purpose

Lentiviral vector - mCherry reporter and sTNFaR for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

Soluble TNFαR (TNFRSF1A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-0K9Q

Plasmid

#221743

Purpose

Stable expression of GFP-0K9Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-1K8Q

Plasmid

#221742

Purpose

Stable expression of GFP-1K8Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-3K6Q

Plasmid

#221741

Purpose

Stable expression of GFP-3K6Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-5K4Q

Plasmid

#221740

Purpose

Stable expression of GFP-5K4Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6

Plasmid

#212820

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-His6 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo(K63R)-6xHis

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K48R)-GFP

Plasmid

#212816

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo(K48R)-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP

Plasmid

#212817

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo(K63R)-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP

Plasmid

#212818

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo(K48R/K63R)-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Stat1Klf6

Plasmid

#188400

Purpose

Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Inserts

Stat-1 (Stat1 Escherichia coli, Mouse)
Klf6 (Klf6 Mouse)

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-hPLXNB2_PGK_Neo

Plasmid

#176848

Purpose

lentiviral vector for constitutive PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Mutation

full length PLXNB2

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCE-hSK

Plasmid

#41814

Purpose

Non-integrating (episomal) expression of human SOX2 and KLF4

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

SOX2, KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

Plasmid

#111731

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q145 (polyQ repeat)

Promoter

CMV and P10

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

Plasmid

#111730

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q109 (polyQ repeat)

Promoter

CMV and P10

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-EF1A>Tet3G:IRES:Neo

Plasmid

#198756

Purpose

Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Tet3G, IRES, Neo

Use

Lentiviral

Promoter

EF1A

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_WT_mCherry

Plasmid

#84020

Purpose

expression of SRSF2 in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP

Plasmid

#183505

Purpose

Express GFP and MAN1C1 proteins

Depositor

Olivier Guipaud

Article

Ladaigue et al iScience. 2022 Nov 2;25(12):105482. doi:

Inserts

man1C1 (MAN1C1 Human)
EGFP

Use

Lentiviral

Tags

Myc-DDK

Expression

Mammalian

Promoter

MND and hPGK

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 NLS1 YFP

Plasmid

#84912

Purpose

Mammalian expresion of TDP-43 NLS1 YFP

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

K82A, R83A, K84A

Promoter

CMV

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_P95H_mCherry

Plasmid

#84023

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95H

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLVET-HA-K-RasG12V-IRES-GFP

Plasmid

#107140

Purpose

A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

HA-tagged Kras4B(G12V) and GFP (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hEF-1alpha

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MCU-Flag-TetOFF

Plasmid

#185657

Purpose

Tet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporter

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

Mitochondrial calcium uniporter (MCU Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

pTight TRE

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-EGFP-PARP1

Plasmid

#176146

Purpose

EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-MCU-mCerulean

Plasmid

#185809

Purpose

Human mitochondrial calcium uniporter with C-terminus mCerulean tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

Mitochondrial calcium uniporter (MCU Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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