We narrowed to 7,680 results for: CCH

Showing: 4701 - 4750 of 7680 results

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pPOM018_P2_pRPL701

Plasmid

#216443

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rpl701

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM017_P2_pTIF51

Plasmid

#216442

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter tif51

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM016_P2_pRPL2501

Plasmid

#216441

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rpl2501

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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IL1_1

Plasmid

#207623

Purpose

Low copy plasmid expressing mTurquoise2 under the TDH3p promoter

Depositor

Yvonne Nygård

Article

Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

Insert

mTurquoise2

Expression

Yeast

Promoter

ScTDH3

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pXOOY

Plasmid

#196449

Purpose

A dual vector for expression of membrane proteins in S. cerevisiae and X. laevis

Depositor

Per Amstrup Pedersen

Article

Vold et al PLoS One. 2023 Feb 21;18(2):e0281868. do

Type

Empty backbone

Use

Expression in xenopus laevis

Expression

Yeast

Promoter

yeast CYC-GAL and T7 promoter

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN100

Plasmid

#205073

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Expression

Bacterial

Mutation

Lacks N Terminal 100 amino acids

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN380

Plasmid

#205076

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Expression

Bacterial

Mutation

Lacks N Terminal 380 amino acids

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN296

Plasmid

#205075

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Expression

Bacterial

Mutation

Lacks N Terminal 296 amino acids

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Atg32 [200-341] 1B

Plasmid

#190431

Purpose

Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_15 [CEN6/ARSH4 ORI]

Plasmid

#198928

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

CEN6/ARSH4 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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Atg20-24 pST39

Plasmid

#200439

Purpose

His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Inserts

Atg20
Atg24

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4-Kina

Plasmid

#202724

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3-EGFP

Plasmid

#201419

Purpose

Bacterial protein expression of Edc3

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Promoter

tac

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2-EGFP

Plasmid

#201417

Purpose

Bacterial protein expression of Dcp2

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Promoter

tac

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3

Plasmid

#201413

Purpose

Bacterial protein expression of Edc3

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp1-EGFP

Plasmid

#201416

Purpose

Bacterial protein expression of Dcp1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3 1-66-EGFP

Plasmid

#201432

Purpose

Bacterial protein expression of Edc3 1-66

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-66 only

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1-EGFP

Plasmid

#201420

Purpose

Bacterial protein expression of Pat1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1

Plasmid

#201414

Purpose

Bacterial protein expression of Pat1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2

Plasmid

#201411

Purpose

Bacterial protein expression of Dcp2

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1-mCh

Plasmid

#201415

Purpose

Bacterial protein expression of Pat1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Tags

MBP, TEV and mCherry, TEV, 6xHis

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp1

Plasmid

#201410

Purpose

Bacterial protein expression of Dcp1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-FLPe-9

Plasmid

#197724

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

FLPe recombinase (tomato optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-R-9

Plasmid

#197726

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

R recombinase (tomato optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpDcp1opt-K44A_AD

Plasmid

#148539

Purpose

Bacterial Expression of SpDcp1opt-K44A

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt-K44A (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpDcp1opt-K44AK47A_AD

Plasmid

#148540

Purpose

Bacterial Expression of SpDcp1opt-K44AK47A

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt-K44AK47A (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpDcp1opt-W107A_AD

Plasmid

#148541

Purpose

Bacterial Expression of SpDcp1opt-W107A

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt-W107A (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpDcp1opt-Y36A_AD

Plasmid

#148542

Purpose

Bacterial Expression of SpDcp1opt-Y36A

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt-Y36A (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpScp1opt-K47A_AD

Plasmid

#148543

Purpose

Bacterial Expression of SpDcp1opt-K47A

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt-K47A (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-SpDcp2opt_1-242_AD

Plasmid

#148545

Purpose

Bacterial Expression of SpDcp2opt_1-242

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242 (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-SpDcp2opt_1-242_AD

Plasmid

#148547

Purpose

Bacterial Expression of SpDcp2opt_1-242

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242 (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-SpDcp2_1-266_X

Plasmid

#148007

Purpose

Bacterial Expression of SpDCP2_1-266

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP2_1-266 (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-SpDCP2_1-242_X

Plasmid

#148008

Purpose

Bacterial Expression of SpDCP2_1-242

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP2_1-242 (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-SpDCP2_1-253_X

Plasmid

#148009

Purpose

Bacterial Expression of SpDCP2_1-253

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP2_1-253 (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpG-SpDCP1_X

Plasmid

#148003

Purpose

Bacterial Expression of SpDCP1

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP1 (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-SpEDC3_1-94_X

Plasmid

#148005

Purpose

Bacterial Expression of SpEDC3_1-84

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpEDC3_1-84 (edc3 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET PI-SceI C-His K403A

Plasmid

#195612

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

Insert

PI-SceI

Expression

Bacterial

Mutation

A1463G, A1464C

Promoter

T7

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7PI-Sce ESARC D326A

Plasmid

#195609

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T3593C, A3601C, T3602G

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SIR4 gRNA HIS

Plasmid

#182520

Purpose

Expression vector for gRNA directed against SIR4 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SIR4 gRNA (SIR4 Budding Yeast)

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CAN1 gRNA HIS

Plasmid

#182521

Purpose

Expression vector for gRNA directed against CAN1 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CAN1 gRNA (CAN1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpG-SpDcp1opt_AD

Plasmid

#148538

Purpose

Bacterial Expression of SpDcp1opt

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-3XHA-eDHFR-Kan

Plasmid

#188928

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Kan

Expression

Yeast

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-DHFR-3xHA

Plasmid

#188934

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-DHFR-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9355(II-1_Markerfree_BackBone)

Plasmid

#161597

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site II-1, (Chr II: 341622..341641)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9356(IV-1_Markerfree_BackBone)

Plasmid

#161598

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IV-1, (Chr IV: 132921..132940)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9360(IX-1_Markerfree_BackBone)

Plasmid

#161601

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IX-1, (Chr IX: 388864..388883)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9362(XV-1_Markerfree_BackBone)

Plasmid

#161603

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XV-1, (Chr XV: 93186..93205)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac211-iGFP-DRS2

Plasmid

#177104

Purpose

Expresses iGFP-Drs2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

DRS2 (DRS2 Budding Yeast)

Expression

Yeast

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB329

Plasmid

#185077

Purpose

NUP82 expression in yeast

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

NUP82

Expression

Yeast

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB280

Plasmid

#185073

Purpose

Larger fusion of Nma111 nuclear localization signal to GFP

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111 aa 1-83

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,680 results for: CCH