We narrowed to 16,177 results for: LEA

Showing: 1881 - 1900 of 16177 results

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NET47 pEGFP-N2 (593)

Plasmid

#61986

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET47 (TM7SF2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
M-ST1n-VP64

Plasmid

#48675

Purpose

Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null (D9A D598A H599A N622A)

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2

Plasmid

#192440

Purpose

Encodes mCherry-tagged wild-type TSC2 isoform 5.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2 (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUB-MSP1D1

Plasmid

#161609

Purpose

Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

UD-MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1 ( Q138W, L181W, Y236W)

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCL1161

Plasmid

#217964

Purpose

For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H3.1 (H3C1 Human)

Tags

eGFP, APEX

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1032

Plasmid

#217962

Purpose

Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

NASP (NASP Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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M-SPn-VP64

Plasmid

#48674

Purpose

Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMBPCas2/3

Plasmid

#89238

Purpose

Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas2/3

Tags

Strep II-MBP

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV HDM2(NES-)

Plasmid

#12087

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

HDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Nuclear-export sequence mutant (NES-): L205A, I20…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
NKP64 GFP (1007)

Plasmid

#62020

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

SLC38A10 (SLC38A10 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pAcGFP-Jpx9C104T

Plasmid

#120381

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

C321T

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD2+pCmYLCV911

Plasmid

#170878

Purpose

Phytobrick - pCmYLCV911

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pCmYLCV911

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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NET39 pEGFP-C1 (1045)

Plasmid

#62024

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET39 (PPAPDC3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pGBW-m4046854

Plasmid

#145759

Purpose

Bacterial Expression plasmid for SARS-CoV-2 leader protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-Z03

Plasmid

#120046

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Jpx9

Plasmid

#120379

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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NET39 mRFP-C1 (1219)

Plasmid

#62042

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET39 (PPAPDC3 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen

Plasmid

#174715

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutations

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

1-332, E151R, R154E

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Jpx9C104A

Plasmid

#120380

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

C321A

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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