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pKRG3-mU6-PUb-hSpCas9-pAc

Plasmid

#162163

Purpose

Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

T2A-pAc

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3 R186S

Plasmid

#175779

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Tags

Expression

Mammalian

Mutation

R186S

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mCit-IRES-Neo

Plasmid

#155230

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mCitrine sensor

Use

Lentiviral

Tags

mCitrine

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

Plasmid

#165430

Purpose

Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

FHS-paCaMKII(SD)

Use

AAV

Tags

FHS(Flag, HisX6, Strep) tag

Expression

Mammalian

Mutation

C450A, L514K, G528A, L531E, N538E: Light-insensit…

Promoter

CaMKII0.4

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

Plasmid

#165429

Purpose

Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

FHS-paCaMKII

Use

AAV

Tags

FHS(Flag, HisX6, Strep) tag

Expression

Mammalian

Mutation

Promoter

CaMKII0.4

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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attB53-Pac-TRE-mCherry-attB53

Plasmid

#183611

Purpose

RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

TRE-mCherry

Use

Rmce

Tags

Expression

Mutation

Promoter

TRE

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{3XP3::DsRed, Pactin::Ptrsps}

Plasmid

#86862

Purpose

Expresses DsRed in eyes and ubiquitous expression of P-transposase

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

pACNNTNPII-S129A

Use

Tags

Expression

Insect

Mutation

S129A

Promoter

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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FRP467-PACT1(-1-520)-LexA-ER-haVP16

Plasmid

#58430

Purpose

Expresses LexA-ER-VP16 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-VP16

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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[pSK24] pACYCDuet His-c7orf59|HBXIP

Plasmid

#136143

Purpose

bacterial expression of c7orf59 and HBXIP

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPD1028 ZF43x6-Compact(SV40Min)_EYFP

Plasmid

#138912

Purpose

EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact(SV40min)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x6-Compact(SV40min)

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-tdTomato-P2A-paCaMKII(K42M)

Plasmid

#165433

Purpose

Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII(K42M)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPD1029 ZF43x6-Compact(CMVMin)_EYFP

Plasmid

#138913

Purpose

EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact(CMVmin)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x6-Compact(CMVmin)

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5 HA3-eDHFR-T2A-puro

Plasmid

#86395

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

Depositor

David Bentley

Article

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A puro

Use

CRISPR

Tags

HA3 eDHFR T2A puro

Expression

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Promoter

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

Plasmid

#217893

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pC009 LshCas13a locus with targeting spacer

Plasmid

#91900

Purpose

LshCas13a locus into pACYC184 with targeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LshCas13a locus with targeting spacer

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pBac{3XP3::EGFP, Pactin::Ptrsps}

Plasmid

#86861

Purpose

Expresses EGFP in eyes and ubiquitous expression of P-transposase

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

pACNNTNPII-S129A

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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PLK1-cjun-FRET-PACT biosensor

Plasmid

#106907

Purpose

centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)

Use

Tags

PACT domain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(K42M)

Plasmid

#165440

Purpose

Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(K42M)

Use

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-tdTomato-P2A-paCaMKII(T286A)

Plasmid

#165432

Purpose

Expresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII(T286A)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

T286A: Autophosphorylation deficient mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP1-Flag-His-7xKQ

Plasmid

#111573

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

lysine to glutamine mutation at the following ami…

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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