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PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

Plasmid

#197989

Purpose

For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNT0217290

Use

Lentiviral

Tags

lac

Expression

Mammalian

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-shRNA2-zsGreen-PGK-puro_scramble shRNA

Plasmid

#197991

Purpose

For the stable knockdown experiments using shRNA, scramble sequence was inserted as the control.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

scramble shRNA (control)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

Plasmid

#167295

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAK (BAK1 Human)

Expression

Mammalian

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG-C-Far

Plasmid

#193914

Purpose

Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-NPRL2

Plasmid

#164985

Purpose

NPRL2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL2 (NPRL2 Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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SuperNova/pRSETB

Plasmid

#53234

Purpose

monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Takeharu Nagai

Article

Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

Insert

monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mouse NT

Plasmid

#242421

Purpose

Expression of a non-targeting shRNA in mouse cells

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

Non-targeting shRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SuperNovaGreen-T2A-dTomato

Plasmid

#193798

Purpose

Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

SuperNova Green

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-GFP-HYGRO

Plasmid

#115144

Purpose

Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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GAL4-DrBphP

Plasmid

#158986

Purpose

Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

GAL4-DrBphP

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_ABE_D3_empty

Plasmid

#203809

Purpose

Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

ABE_D3

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-RFP-HYGRO

Plasmid

#115145

Purpose

Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

RFP

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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LDB-14-p65

Plasmid

#158985

Purpose

Expresses LDB-14 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-14-p65

Tags

V5 tag , 6xHis tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Keap1

Plasmid

#62454

Purpose

His6 tagged Keap1 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Tag

Expression

Bacterial

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-Puro

Plasmid

#115143

Purpose

Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Puromycin

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVhPOP1_3Flag

Plasmid

#53968

Purpose

Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

POP1 (POP1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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