159,557 results

1. PL-CRISPR.EFS.tRFP-gIfngr1-2

   Plasmid

   #166480

   Purpose

   Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   gRNA for mouse Ifngr1 (Ifngr1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR EGFR-CD3ε-FusionRed

   Plasmid

   #188626

   Purpose

   Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   CD3ε-FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH6HTC_TKT-Halo

   Plasmid

   #175331

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   TKT (TKT Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKL2315

   Plasmid

   #187876

   Purpose

   Expresses Cre recombinase.

   Depositor

   Kan Wang

   Article

   Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23107

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKIIa-hChR2(H134R)-EYFP

   Plasmid

   #26969

   Purpose

   AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

   Has Service

   AAV1, AAV2, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Histidine 134 is mutated to Arginine

   Promoter

   CaMKIIa

   Available since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. RepairTemplate_NIPBL_mEGFP_mAID

   Plasmid

   #217664

   Purpose

   Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   NIPBL_mEGFP_mAID (NIPBL Human)
   

   Use

   Tags

   mAID and meGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

   Plasmid

   #217662

   Purpose

   expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Talin head-SpyCatcher003

   Plasmid

   #133566

   Purpose

   Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   EGFP-Talin head-SpyCatcher003
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. cfSGFP2-N

   Plasmid

   #37533

   Depositor

   Ikuo Wada

   Article

   Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

   Insert

   cysteine-free SGFP2-N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C48S and C70M mutations relative to SGFP2

   Promoter

   CMV

   Available since

   Aug. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLGR002 LGR Cas9 guide vector

    Plasmid

    #188320

    Purpose

    Universal backbone for CRISPR/Cas9 sgRNA expression

    Depositor

    Laralynne Przybyla

    Article

    Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    PuroR-T2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    pU6 5'CMV and EF1-alpha

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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