We narrowed to 31,920 results for: ica
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Plasmid#47457PurposeLITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.DepositorInsertNLS(alpha-imp)-CRY2PHR-NLS-VP64
Tags2A_GFPExpressionMammalianPromoterEF1-alphaAvailable SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX
Plasmid#182063PurposeCo-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)DepositorInsertQuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX
UseLentiviralPromoterCAGAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II KS(+) EACMCV DNA-A 1.6mer
Plasmid#159136PurposeInfectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 1998 DNA-ADepositorInsertEACMCV DNA-A 1.6mer
UseInfectious clone for bombarding plantsAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM8
Plasmid#220166PurposeExpresses a reporter for an alpha-helical OMM proteinDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CAAX
Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialPromoteraraBADAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXK-1/1
Plasmid#91539PurposeProtein expression and purification of human SH3 domain construct TXK-1/1DepositorInsertTXK-1/1 (TXK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
ExpressionBacterialMutationLysine 622 was replaced by AlanineAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+PIF6CreN
Plasmid#131780PurposeMammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite systemDepositorInsertPIF6CreN
UseCre/Lox; Zebrafish expressionExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+PhyBΔCreC
Plasmid#131781PurposeMammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.DepositorInsertPhyBCreC
UseCre/Lox; Zebrafish expressionExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDuex2-iBB-GECO1
Plasmid#194216PurposeFar-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.DepositorInsertiBB-GECO1
Tags6xHisExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule2-IBBN (G2)
Plasmid#85501PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OTmut
Plasmid#185389PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVPromoterEFSAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf170
Plasmid#12858PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf170
ExpressionMammalianAvailable SinceDec. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cTRb413
Plasmid#188261PurposePlasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterChicken B-ActinAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
Plasmid#199585PurposeExpress tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf106
Plasmid#12794PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf106
ExpressionMammalianAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pME-CreLite
Plasmid#131782PurposeMiddle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.DepositorInsertPhyBCreC-P2A-PIF6CreN
UseCre/Lox; Gateway cloningAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SKAP2-1/1
Plasmid#91447PurposeProtein expression and purification of human SH3 domain construct SKAP2-1/1DepositorInsertSKAP2-1/1 (SKAP2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf45
Plasmid#12733PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf45
ExpressionMammalianAvailable SinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CreLite
Plasmid#131786PurposeLentiviral vector expressing CreLite, driven by CBh promoterDepositorInsertPhyBCreC-P2A-PIF6CreN
UseCre/Lox, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterCBhAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf40
Plasmid#12728PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf40
ExpressionMammalianAvailable SinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf107
Plasmid#12795PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf107
ExpressionMammalianAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CDC10
Plasmid#51568PurposeConstitutively express bud neck localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsCDC10 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]
Plasmid#66781PurposeGly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOGDepositorInsertminiSOG
Tagstomm-20 (N terminal 55 aa)ExpressionWormMutationG426CAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47454PurposeepiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.DepositorInsertCRY2PHR-NLS-SID4X
UseAAVTags2A_phiLOV2.1ExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDule-IBBN (G2)
Plasmid#85500PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBASH3B-1/1
Plasmid#91462PurposeProtein expression and purification of human SH3 domain construct UBASH3B-1/1DepositorInsertUBASH3B-1/1 (UBASH3B Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2VL-IRES-dOrai
Plasmid#67629PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorInsertdmBACCS2VL-IRES-dOrai
ExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRY2-mCherry-iTrkA
Plasmid#106168Purposemammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)DepositorAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Spc72
Plasmid#51582PurposeConstitutively express spindle pole body localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsSpc72 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PIK3R2-1/1
Plasmid#91415PurposeProtein expression and purification of human SH3 domain construct PIK3R2-1/1DepositorInsertPIK3R2-1/1 (PIK3R2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
HEpl017
Plasmid#179992PurposepCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoterDepositorInsertTadA8e-dSiCas12i(D1049A)
ExpressionMammalianAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniSOG-T2A-dTomato
Plasmid#193796PurposeExpresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomatoDepositorInsertminiSOG
UseAAVTagsFlag tagExpressionMammalianPromoterCAGAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NOXO1-1/2
Plasmid#91499PurposeProtein expression and purification of human SH3 domain construct NOXO1-1/2DepositorInsertNOXO1-1/2 (NOXO1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf42
Plasmid#12730PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf42
ExpressionMammalianAvailable SinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_OTmut
Plasmid#185385PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cellsDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
ExpressionMammalianPromoterCMVAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only