We narrowed to 3,255 results for: actin

1. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

   Plasmid

   #211817

   Purpose

   AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   N-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter and U6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

   Plasmid

   #211818

   Purpose

   AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   C-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter and U6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACT1:Cas9-GFP, U6:sgTK

   Plasmid

   #122852

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgTK
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACT1:Cas9-GFP, U6:sgUPRT

   Plasmid

   #122853

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgUPRT
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa-FRB-3myc-KIF1A tail 391-1698

   Plasmid

   #64286

   Purpose

   KIF1A aa 391-1698 with N-terminal FRB and triple myc tags

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   KIF1A (Kif1a, Kif1a Rat, Mouse)
   

   Tags

   3myc and FRB

   Expression

   Mammalian

   Mutation

   aa 391-1698, 5'end mostly matches rat, 3…

   Promoter

   chicken Beta Actin

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIG-VP16–RAR-alpha

   Plasmid

   #80434

   Purpose

   Expression of constitutively active human RAR-alpha in chick embryos

   Depositor

   Aixa Morales

   Article

   Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

   Insert

   Retinoic acid receptor alpha (RARA Human)
   

   Tags

   EGFP and VP16

   Expression

   Mammalian

   Promoter

   beta-actin promoter

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pCAGGS-Twin-Strep-TEV-DGKζ

   Plasmid

   #202531

   Purpose

   Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   DGKZ (DGKZ Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMYC BRGI aa597-1407

   Plasmid

   #19146

   Depositor

   Joan Massague

   Article

   Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   human BRG1 aa 597-1407 (PCR product)

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

   Plasmid

   #36881

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   tdT-3Myc
   insulator
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cav2.2 D122A HA pCAGGS

    Plasmid

    #206097

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV/B-actin

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. UPRT-mCh-Nluc-P2A-neo-UPRT

    Plasmid

    #135015

    Purpose

    Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    mCh-Nluc-P2A-neo
    UPRT 5'UTR
    UPRT 3'UTR
    

    Use

    Cryptosporidium expression

    Tags

    mCherry and Nluc-neo

    Promoter

    Cryptosporidium actin and enolase

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMYC BRGI aa1400-1648

    Plasmid

    #19145

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    human BRG1 aa 1400-1648 (PCR product)

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

13. Lenti CGIP + M-AAT target

    Plasmid

    #86008

    Purpose

    lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    M-AAT target
    

    Use

    Lentiviral

    Mutation

    V30M

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV hGRN4

    Plasmid

    #213684

    Purpose

    Expresses human granulin-4 with an N-terminal twin-Strep-FLAG tag

    Depositor

    Thomas Kukar

    Article

    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

    Insert

    Human granulin-4 (hGRN4) (GRN Human)
    

    Use

    AAV

    Tags

    Twin-Strep tag and FLAG tag (after signal peptide…

    Promoter

    cytomegalovirus enhancer/chicken β-actin promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid

    #211821

    Purpose

    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter, U6 promoter and hPGK pr…

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-SMSr-TEV-Twin-Strep

    Plasmid

    #202530

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SAMD8 (SAMD8 Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TGA)

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti CGIP + Z-AAT target

    Plasmid

    #86007

    Purpose

    lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    Z-AAT target
    

    Use

    Lentiviral

    Mutation

    V30M

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti CGIP + TTR target

    Plasmid

    #86009

    Purpose

    lenti reporter plasmid with TTR_V30M-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    TTR target
    

    Use

    Lentiviral

    Mutation

    V30M

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCYP16

    Plasmid

    #212849

    Purpose

    Expresses the Actinomadura pelletieri LRD-modifying cytochrome P450 enzyme

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Insert

    cytochrome P450
    

    Expression

    Bacterial

    Promoter

    PcymRC

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCYP17

    Plasmid

    #212850

    Purpose

    Expresses the Actinomadura sp. KC06 LRD-modifying cytochrome P450 enzyme

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Insert

    cytochrome P450
    

    Expression

    Bacterial

    Promoter

    PcymRC

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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