159,557 results

1. pAAV.CMV.ffLuciferase.SV40

   Plasmid

   #105532

   Purpose

   AAV expression of firefly luciferase from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   Luciferase
   

   Use

   AAV and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMV306G13+FFluc

   Plasmid

   #26157

   Depositor

   Siouxsie Wiles, Brian Robertson

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   G13 promoter + Firefly luciferase
   

   Use

   Mycobacteria integrating vector

   Tags

   Expression

   Mutation

   Codon optimized for Mycobacterium tuberculosis. …

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-5HRE-GFP NeoR

   Plasmid

   #128960

   Purpose

   Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   5X HRE of VEGF (VEGFA Human)
   

   Use

   Lentiviral

   Tags

   d2EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKT-IDH1(R132H)-IRES-Katushka

   Plasmid

   #124257

   Purpose

   Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

   Insert

   Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Arginine 132 is mutated to Histidine. This mutati…

   Promoter

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-SLC25A12_STOP

   Plasmid

   #161104

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A12 (SLC25A12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

   Plasmid

   #225756

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. UbC-Halo-SFPQ

    Plasmid

    #166946

    Purpose

    Lentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    Halo

    Expression

    Mutation

    Promoter

    UbC

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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