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MTK1_032

Plasmid

#123694

Purpose

Encodes ConL3 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_033

Plasmid

#123695

Purpose

Encodes ConL4 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_034

Plasmid

#123696

Purpose

Encodes ConL5 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_003

Plasmid

#123698

Purpose

Encodes pUAS (miniSV40 core) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pUAS (mini SV40)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_012

Plasmid

#123675

Purpose

Encodes ConL4 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Phhf2-PYC-Tadh1

Plasmid

#127737

Purpose

Yeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

Phhf2

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[DsxF].1026A

Plasmid

#112690

Purpose

express gRNA targeting DsxF under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Tra].1046D

Plasmid

#112693

Purpose

express two gRNA targeting bTub & Tra under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mei-S332 5.6Kb genomic DNA in Casper4

Plasmid

#112984

Purpose

P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo) (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Prps3-CAD-Tcyc1

Plasmid

#127728

Purpose

Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prps3

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Peft2-CAD-Tklura3

Plasmid

#127729

Purpose

Yeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Peft2

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Psmtef1-CAD-Tyhi9

Plasmid

#127730

Purpose

Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psmtef1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Psptdh3-ACO-Tagtef1

Plasmid

#127734

Purpose

Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psptdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Ppgk1-PYC-Ttpi1

Plasmid

#127735

Purpose

Yeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppgk1

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Ppxr1-PYC-Tnat1

Plasmid

#127736

Purpose

Yeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppxr1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Psbtdh3-CSC-Tefm1

Plasmid

#127742

Purpose

Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psbtdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Peno2-ACDH-Ttal1

Plasmid

#127743

Purpose

Yeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Peno2

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Pvma6-ACDH-Trps14a

Plasmid

#127744

Purpose

Yeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pvma6

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Prpl28-ACDH-Tecm10

Plasmid

#127745

Purpose

Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prpl28

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Ptdh3-ACDH-Trpl3

Plasmid

#127746

Purpose

Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eB

Plasmid

#103005

Purpose

non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.eB VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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rAAV2/MG1.2

Plasmid

#184541

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.2 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, Q588T; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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rAAV2/MG1.1

Plasmid

#184540

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.1 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, A587L, Q588M; inse…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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wt Opa1

Plasmid

#204427

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3A

Plasmid

#241246

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) (BLTP3A Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Control-1

Plasmid

#204442

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L243A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmKate1-N1

Plasmid

#31878

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pMT525

Plasmid

#131392

Purpose

L1RP retrotransposition reporter

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP GFP-AI

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3_CR

Plasmid

#108104

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation)

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

Plasmid

#42335

Purpose

A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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