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Wnt2 STOP

Plasmid

#35869

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Use

Entry vector

Mutation

Silent Mutation G (816) --> A

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2H2D

Plasmid

#67081

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H2D (GTF2H2C_2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJUMP18-Streptag_C

Plasmid

#127036

Purpose

Basic Part C- C-terminus; Strep-tag II

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_C

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_CMVenhancer

Plasmid

#62419

Purpose

MXS_chaining vector with CMVenhancer

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV enhancer

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pKFSS1_Psyn-sgRNA500

Plasmid

#149557

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn and pSyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_MCS2

Plasmid

#62396

Purpose

MXS_chaining vector with MCS2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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Wnt3A Non-STOP

Plasmid

#35890

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFGL1252

Plasmid

#118991

Purpose

A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Type

Empty backbone

Use

Agrobacterium tumerfaciens-mediated transformation

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-mCherryBb

Plasmid

#149636

Purpose

Psyn-mCherry promoter strength reporter for use in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

Psyn-mCherryBb

Tags

mCherry

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 RAP2A

Plasmid

#67058

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RAP2A (RAP2A Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJUMP19-L3S1P51_T

Plasmid

#127037

Purpose

Basic Part T- Terminator; L3S1P51; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pbad_P

Plasmid

#127003

Purpose

Basic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose induced; controlled by AraC.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pbad

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-T7pro_P

Plasmid

#127002

Purpose

Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part T7 promoter_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-CBh-Cas9n-pA

Plasmid

#82617

Purpose

Expresses Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

SpCas9n

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_N

Plasmid

#127034

Purpose

Basic Part N- N-terminus; Strep-tag II

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_EF1a

Plasmid

#62421

Purpose

MXS_chaining vector with EF1a

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EF1a Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-Dnm2a

Plasmid

#109571

Purpose

Multisite gateway vector for 3' tagging with Dynamin2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2a

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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RALB

Plasmid

#55658

Purpose

expression of mouse RALB

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RALB (Ralb Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pRS306Sup35R1+2

Plasmid

#17716

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+2 (SUP35 Budding Yeast)

Expression

Yeast

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
PP165

Plasmid

#78985

Purpose

Supplemental Figure 4. Promoter GCW14. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1674-HQ858865

Plasmid

#27947

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bHLH transcription factor encoded by Os08g0490000 LOC_Os08g38210

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pET11a+D77R/T214V

Plasmid

#66135

Purpose

Expresses the D77R/T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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Wnt5B STOP

Plasmid

#35875

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
AAA

Plasmid

#78487

Purpose

2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail

Depositor

Allan Jacobson

Article

Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d

Insert

CAN1-LUC

Expression

Bacterial

Mutation

CAN1 contains no stop codon

Available Since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only
pHYRSF236

Plasmid

#46377

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaE intein delta variant, inactive

Tags

H6-Smt3

Expression

Bacterial

Mutation

C1A, C+1A, deleted T76, V97, D98, L100 from NpuDn…

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt8B-V5

Plasmid

#35936

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1183-HQ858805

Plasmid

#27887

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

ORPHAN transcription factor encoded by Os02g0606200 LOC_Os02g39360

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1184-HQ858806

Plasmid

#27888

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bZIP transcription factor encoded by Os05g0129300 LOC_Os05g03860

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1185-HQ858807

Plasmid

#27889

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bZIP transcription factor encoded by Os07g0687700 LOC_Os07g48820

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP47ts-2A(sfGFP)

Plasmid

#126992

Purpose

Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_PGKenhancer

Plasmid

#62416

Purpose

MXS_chaining vector with PGKenhancer

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PGK enhancer

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L3S1P51_CT

Plasmid

#127047

Purpose

Basic Part CT- C-terminus + Terminator; L3S1P51. C-termini STOP + terminator (synthetic)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-mCherry_O

Plasmid

#127025

Purpose

Basic Part O- ORF; mCherry; fluorescent protein

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part mCherry_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23107_P

Plasmid

#127000

Purpose

Basic Part P- Promoter (constitutive); J23107.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23107_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23100_P

Plasmid

#126996

Purpose

Basic Part P- Promoter (constitutive); J23100

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23100_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript_VEGFA-TS3_SpCas9

Plasmid

#107328

Purpose

U6 driven SpCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript_VEGFA-TS3_NmCas9

Plasmid

#107329

Purpose

U6 driven NmCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-p85α-ΔiSH2-N

Plasmid

#39498

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-N (PIK3R1 Bovine)

Use

Eukaryotic

Mutation

ΔiSH2-N = deletion of AA478-513

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T H42Q

Plasmid

#9065

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
PP275

Plasmid

#78968

Purpose

Promoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
-226bp goosecoid luciferase

Plasmid

#17158

Depositor

Ken Cho

Article

Watabe et al Genes Dev. 1995 Dec 15. 9(24):3038-50.

Insert

goosecoid promoter (gsc-a Frog)

Use

Luciferase

Tags

luciferase

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pGEM cCD44rel (CT#690)

Plasmid

#13946

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

CD44 related (CD44 Chicken)

Expression

Bacterial

Mutation

Nucleotides 814-1284

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRS406 ATG9-GFP S122D

Plasmid

#114667

Purpose

Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

changed serine 122 to aspartic acid

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
RHEBL1

Plasmid

#55684

Purpose

expression of mouse RHEBL1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RHEBL1 (Rhebl1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
EC.RNe.TYB1.a1-400.wt

Plasmid

#17949

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-400

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-400

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pUT1858-HQ858878

Plasmid

#27960

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bHLH transcription factor encoded by Os01g0928000 LOC_Os01g70310

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-EPN-01(ΔL2)

Plasmid

#80050

Purpose

Expression of EPN-01 mutated in ALIX binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaYP)

Tags

Myc

Expression

Mammalian

Mutation

YP37AA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode83

Plasmid

#83760

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode83

Use

Retroviral

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GST HP1 gamma deltaC

Plasmid

#24077

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

GST

Expression

Bacterial

Mutation

Missing the C-terminal 25 amino acids

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

Showing: 4801 - 4850 of 17288 results

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